TABLE 1

Source materials of cDNA libraries and numbers and characteristics of EST clones

No. ofMost frequent
 junk
Library
 designationaGenotypeTissueStagebConditionClonesESTsIn
 contigscUnique contigsdSinglesUnigenes/cloneUnique/cloneeJunkf
 (%)Sourceg%
AS040E1XF2 from crossAntherPremeiotic2,5703,3591,0452461,3760.9420.631 1.21TREP0.35
AS067E1XF2 from crossAntherPremeiotic1,0681,068445194250.8150.416 2.20TREP0.82
SC010XXXBlancoRoot tipSeedlingAluminum1,2291,229361397010.8640.602 0.24TREP0.08
SC013XXXBlancoRoot tipSeedling894894265215540.9160.643 0.33Chimera0.22
SC024E1XBlancoAntherDeveloping spike6,5537,6791,6458932,9760.7050.590 0.98rRNA0.45
TA001E1SCheyenneEndosperm5–30 DAP2222221225700.8650.338 2.20Mitochon.0.88
TA001E1XCheyenneEndosperm5–30 DAP3,1113,1111,222727530.6350.265 2.63rRNA1.91
TA001E2NCheyenneEndosperm5–30 DAP6565360280.9850.43190.75Sorghum90.33
TA005E1XChinese SpringRoot and shootSeedlingDrought875971540293150.9770.393 1.02Chimera0.51
TA005E2SChinese SpringRoot and shootSeedlingDrought828828403562200.7520.333 2.36E. coli0.83
TA006E1XChinese SpringShootSeedlingEtiolated2,7283,0211,3951327570.7890.326 3.27Mitochon.1.76
TA006E2NChinese SpringShootSeedlingEtiolated1,5081,6225301281480.4500.183 2.41rRNA1.08
TA006E3NChinese SpringShootSeedlingEtiolated657715420322140.9650.37465.79Sorghum64.07
TA007E1XChinese SpringRoot and shootSeedlingCold1,0331,333671594441.0790.487 1.91TREP0.44
TA007E2SChinese SpringRoot and shootSeedlingCold491534223312060.8740.48323.82E. coli19.69
TA007E3SChinese SpringRoot and shootSeedlingCold491507232241820.8430.42091.05Sorghum90.02
TA008E1XChinese SpringRootSeedlingEtiolated3,6344,1692,0081691,1930.8810.375 0.95rRNA0.24
TA008E3NChinese SpringRootSeedlingEtiolated4,4505,3172,2455799040.7080.333 2.60E. coli0.77
TA012XXXBrevorEmbryoDormant seedABA1,7671,76788987610.9340.43517.58rRNA16.23
TA015E1XChinese SpringRoot and shootSeedlingHeat1,3391,652734436301.0190.503 1.73Mitochon.0.54
TA016E1XChinese SpringCrownSeedlingCold2,7893,5131,6421631,1741.0100.479 1.01rRNA0.31
TA017E1XChinese SpringSpike20–45 DAP1,1671,317523322720.6810.260 0.98rRNA0.38
TA018E1XChinese SpringSpike5–15 DAP2,9123,5351,6091231,1860.9600.450 0.31TREP0.23
TA019E1XChinese SpringSpikePreanthesis12,19415,3165,3447905,6980.9060.532 0.65TREP0.23
TA027E1XTAM W101LeafFull tilleringDrought1,3331,486674524990.8800.413 1.78Mitochon.0.93
TA027E2STAM W101LeafFull tilleringDrought991991523143610.8920.378 1.88Chloroplast0.69
TA031E1XChinese SpringLeafFull tilleringHeat1,1771,530724705171.0540.499 1.29TREP0.84
TA032E1XChinese SpringSpike5–20 DAPHeat1,1801,506684495711.0640.525 0.86rRNA0.33
TA036E1XChinese SpringLeafFull tilleringDrought787981456303761.0570.516 2.29TREP0.60
TA037E1XChinese SpringSheathSheathSalt9771,242613473600.9960.417 1.90rRNA0.39
TA038E1XChinese SpringCrownCrownSalt1,2411,537811544991.0560.446 1.35rRNA0.45
TA047E1XChinese SpringRoot tipRoot tip1,1571,210688452780.8350.279 0.98E. coli0.33
TA048E1XBH1146Root tipRoot tipAluminum1,0161,069565323290.8800.355 0.56Chimera0.37
TA049E1XBrevorEmbryoDormant seedDormant3,1383,2831,4111149900.7650.352 6.12rRNA4.83
TA055E1XChinese SpringRootFull tilleringDrought1,3261,326686155080.9000.394 1.41TREP0.74
TA056E1XChinese SpringRoot tipSeedlingAluminum1,2081,208719283210.8610.289 0.66Chimera0.33
TA058E1XChinese SpringRootFull tillering1,0241,02458383570.9180.356 1.73TREP0.86
TA059E1XButte 86Grain3–44 DAPVarious3,7083,7081,674617850.6630.228 3.13rRNA1.49
TA065E1XChinese SpringRootFull tilleringSalt2,1292,1291,153267380.8880.359 1.16  Chimera0.70
TA066E1XChinese SpringAllFull tillering1,4121,412767104340.8510.314 0.77  TREP0.28
TM011XXXDV92Shoot apexVegetative3,1883,1881,3181311,1940.7880.416 2.92  TREP1.83
TM043E1XDV92Shoot apexReproductiveVernalized2,8023,5901,3493071,1430.8890.517 3.65  TREP1.88
TM046E1XG3116Shoot apexReproductiveVernalized3,4713,4711,3081431,2950.7500.414 2.72  TREP1.51
TT039E1XLangdon-16AllVarious1,2031,472684714710.9600.451 0.67  rRNA0.20
Total89,043101,10716,7405,00033,2130.5610.429
  • a The species source of the library is indicated by the first two letters of the designation: TA, T. aestivum; TM, T. monococcum; TT, T. turgidum; SC, Secale cereale; and AS, A. speltoides. Any enrichment technique applied is indicated by the last character in the designation: N, normalized and S, subtracted.

  • b DAP, days after pollination.

  • c In contigs, number of contigs in which sequences from this library participate. The total is not the sum of the column, but the total number of contigs when considering all of the libraries at once.

  • d Unique contigs, those that are composed of sequences only from this library.

  • e Unique/clone, (unique contigs + singles)/number of clones.

  • f Percentage of library composed of clones other than the intended cDNAs.

  • g TREP, Triticeae Repeat Sequence Database (http://wheat.pw.usda.gov/ggpages/ITMI/Repeats/index.shtml); Mitochon., Mitochondrion.