TABLE 1

Strains and plasmids used in this study

GenesDescriptionReference
DR0003ddrC (DNA damage response C)This study
DR0070ddrBThis study
DR0326ddrDThis study
DR0423ddrAHarris et al. (2004)
DRA0346pprAAccession no. O32504
Strains
 Deinococcus radiodurans R1ATCC13939
 TNK101As R1 but ΔddrC::pkat-aadAThis study
 TNK102As R1 but ΔddrB::pkat-catThis study
 TNK103As R1 but ΔddrD::pkat-kanThis study
 TNK104As R1 but ΔddrA::pkat-hygHarris et al. (2004)
 TNK105As R1 but ΔpprA::pkat-aadAThis study
 TNK106As R1 but ΔrecA::pkat-catHarris et al. (2004)
 TNK107ΔddrC::pkat-aadA, ΔrecA::pkat-catThis study
 TNK108ΔddrB::pkat-aadA, ΔrecA::pkat-catThis study
 TNK109ΔddrD::pkat-kan, ΔrecA::pkat-catThis study
 TNK110ΔddrA::pkat-hyg, ΔrecA::pkat-catHarris et al. (2004)
 TNK111ΔpprA::pkat-aadA, ΔrecA::pkat-catThis study
 TNK112ΔddrB::pkat-cat, ΔddrC::pkat-aadA,This study
 TNK113ΔddrC::pkat-aadA, ΔddrD::pkat-kanThis study
 TNK114ΔddrA::pkat-hyg, ΔddrC::pkat-aadAThis study
 TNK115ΔddrC::pkat-aadA, ΔpprA::pkat-hygThis study
 TNK116ΔddrB::pkat-cat, ΔddrD::pkat-kanThis study
 TNK117ΔddrA::pkat-hyg, ΔddrB::pkat-catThis study
 TNK118ΔddrB::pkat-cat, ΔpprA::pkat-hygThis study
 TNK119ΔddrA::pkat-hyg, ΔddrD::pkat-kanThis study
 TNK120ΔddrD::pkat-kan, ΔpprA::pkat-aadAThis study
 TNK121ΔddrA::pkat-hyg, ΔpprA::pkat-aadAThis study
 TNK122As R1 but ΔddrB::pkat-aadAThis study
 TNK123As R1 but ΔpprA::pkat-hygThis study
 DH5α-MCRF mcrA Δ(mrr-hsdRMS-mcrBC)
   Φ80 lacZΔ15 ΔlacX74 endA1
   recA1 deoR Δ(ara-leu) 7697
   araD139 galU galK nupG rpsLInvitrogen (Grand Island, NY)
Plasmids
 pGEM-TTA cloning vectorPromega (Madison, WI)
 pTNK101pGEM-T::pkatA-catThis study
 pTNK102pGEM-T::pkatA-kanThis study
 pTNK103pGEM-T::pkatA-aadAThis study
 pTNK104pGEM-T::pkatA-hygThis study
 pTNK201pGEM-T::ΔddrC::pkatA-aadAThis study
 pTNK202pGEM-T::ΔddrB::pkatA-catThis study
 pTNK203pGEM-T::ΔddrB::pkatA-aadAThis study
 pTNK204pGEM-T::ΔddrD::pkatA-kanThis study
 pTNK205pGEM-T::ΔddrA::pkatA-hygHarris et al. (2004)
 pTNK207pGEM-T::ΔpprA::pkatA-aadAThis study
 pTNK208pGEM-T::ΔpprA::pkatA-hygThis study
 pTNK210pGEM-T::ΔrecA::pkatA-catHarris et al. (2004)