TABLE 1

A set of candidate genes detected by AFLP-cDNA display shows differential expression patterns in new A. suecica lines

Clone IDPutative
 origin of
 silencingAt2At4AaS2S3S4S5AsGene IDGene name
E-AC/1/2X/1 At++0+++0F24J35.19Peptide transporter
E-AC/3/2X/1 At++00000F617.9Protein transporter (PT)
E-AC/4/2X/1 At++T9N14.8Cryptozoic factor, putative
E-AC/4/2X/2 At++0+++AT4G28710Myosin heavy chain-like protein
E-AC/8/2X/1 At++F22C12.7Hypothetical protein
E-AG/6/2X/1 At++++F1M20.33Myb-like DNA-binding domain
E-AT/1/2X/1 At+++++F28C11Similarity to peroxidase isozyme
E-AT/1/2X/2 At++0T27G7.16Unknown protein
E-AT/7/2X/3 At++0++0+0F19K23.19Similar to peroxidase isozyme
E-TT/2/2X/1 At++MYH9.6Putative protein
E-TT/2/2X/2 At++0+F26P21.170Putative protein (PP1)
E-TT/2/2X/3 At++00F6N7.24Putative protein
E-AC/7/4X/1 At++00000T21H19.200Putative protein KIAA1012 protein
E-AT/1/4X/2 At+++++T23K8.10Similarity to serine threonine kinase (STK)
E-TC/4/2X/1 At+++++MLJ15.9Light-regulated protein
E-AC/7/F3/1 At++00+0+F7A10.1Unknown protein
E-AC/5/2X/1 At+0++++++MYH9.8Cellulose synthase catalytic subunit
E-AT/1/2X/3 At+F17I14.150NAD-dependent malate dehydrogenase
E-TG/6/2X/1 At++++++T25K16.6DNA-binding protein (DBP)
E-AG/6/2X/3 At+++++0++F14N12.2Putative lipase
E-AA/5/CA/1 At+++F22D16.7Glutathione S-transferase (GST)
E-TG/6/CA/1 At++++++F10M23.190Putative protein (PP2)
E-TT/4/4X/1 At0++++++MBM17.52Similarity to SNF2/RAD54 family (RAD54)
E-AC/5/4X/2 At0+0++++T4O12.9Vacuolor sorting protein 35
E-AT/6/4X/2 At0+++++++MPA24.12Nuclear matrix constituent protein 1
E-TA/6/4X/1 At0++0++++F16L2.60Kinesin-related protein
E-AG/3/CA/1 Aa00+++000F10K1.19Fructose-2,6-bisphosphatase
E-TG/1/CA/1 Aa00++0000F5M15.5Hypothetical protein
E-AA/3/CA/1 Aa00++++0K14A17.7E2, ubiquitin-conjugating enzyme
E-AA/4/CA/1 Aa+++MJL12.21Rotamase FKBP
E-AT/7/CA/3 Aa00++0+0+T24D18.11Unknown protein
E-TC/2/CA/1 Aa00+++000T9J14.13Putative ribosomal protein
E-TG/2/CA/1 Aa00++0+++F1B16.11Chloroplast 50S ribosomal protein
E-TG/3/CA/1 Aa00+++++0T9G5.7Copia-type polyprotein
E-AC/8/F2/2 Aa00+++++0F1P2.90Endochitinase-like protein
E-AC/7/Se/2 Aa00+00+++F2A10.1Unknown protein
E-AA/5/F2/2 Novel++F5A8.1Similar to Rubisco small unit
E-TC/3/F2/1 Novel000++++0MCO15.7Putative protein
E-TC/3/F2/2 Novel000++0+0T22P15.2Hypothetical protein
E-TG/1/F2/2 Novel000++++LHI6299Similarity to recN gene
E-TT/3/F2/1 Novel000++++0MKP6.31Hypothetical protein
E-TT/3/F2/2 Novel000++000T3K9.18Hypothetical protein
E-TT/1/F3/1 Novel0000+000T27G7.20Mg-chelatase, putative
  • At, A. thaliana; At2, A. thaliana diploid; At4, A. thaliana autotetraploid; Aa, A. arenosa; As, A. suecica; S2–5, selfing generations 2, 3, 4, and 5; Novel, expression is present in polyploids but absent in either parent; 0, −, and + indicate no expression, very low, and very high levels of expression detected, respectively.