TABLE 4

Microtubule staining reveals mitotic spindles in wild-type tetraploids, but not in haploids, cln3 tetraploids, or tetraploids incubated in water

Strain% cells with mitotic
 spindles/total cells
MATα WT 1.8 ± 1.2
MATα/α/α/α WT18.5 ± 3.7
MATα/α/α/α, 2μ-MATa 7.2 ± 1.9
MATα, H2O 1.6 ± 0.5
MATα/α/α/α, H2O 2.3 ± 1.9
MATα, cln3 1.9 ± 0.3
MATα/α/α/α, cln3/cln3/cln3/cln3 5.4 ± 2.3
  • Comparison of mitotic spindle formation in haploids and tetraploids. Wild-type tetraploids show a significantly large percentage of mitotic spindles relative to wild-type haploids, cln3 tetraploids, and tetraploids incubated in water. All strains were grown for 7 days in stationary phase. WT, wild type.