TABLE 5

Gene targeting rates

Gene	FlyBase	Site	Arm	BG rate	KG rate	EY rate	EP rate	PA/PC  rate
KG hotspots (22F–23A)
CG9894		23A3	2L	20	996	19	45	0
CG16987	Alp23B	23B1	2L	0	89	10	5	0
CG9884	oaf	22F3	2L	0	95	15	0	0
CG3539	Slh	22F3	2L	0	24	0	0	0
CG3104		23B5	2L	0	21	1	0	0
CG31690	CG31690	23A2	2L	0	16	0	0	0
KG hotspots (38B–44A)
CG8676	Hr39	39B4	2L	35	246	15	10	20
CG11546	l(2)02045	44B7	2R	15	136	4	5	0
CG8709		44B5	2R	0	96	8	5	0
CG31611/  CG31613		39E1	2L	0	80	11	0	0
CG8678		39B3	2L	0	45	3	0	0
CG8677	BEST:LD14959	39B3	2L	0	39	1	5	0
CG15845	Adf1	42C3	2R	0	34	8	10	0
CG31626		39B4	2L	5	29	1	0	10
CG2163	Pabp2	44B4	2R	0	28	4	5	0
CG12110	Pld	42A15	2R	0	26	5	0	0
CG9243/  CG9244	Acon	39A7	2L	0	21	1	0	0
CG10718	neb	38B4	2L	5	19	5	5	0
CG10746	fok	38B4	2L	5	19	5	0	0
KG hotspots (49B–F)
CG4654	Dp	49F10	2R	0	38	5	5	0
CG4670		49F11	2R	0	24	0	0	0
CG4663		49F11	2R	0	13	0	0	10
EY/EP/BG hotspots (85C–91F)
CG9429	Crc	850E1	3R	55	5	31	25	0
CG10120	Men	87C6	3R	15	9	46	30	0
CG5555/  CG31475		91F11	3R	0	8	29	60	0
CG11033		85C3	3R	15	4	30	35	0
CG3937	cher	89E13	3R	40	5	20	5	0
CG9366	RhoL	85D18	3R	0	5	16	15	0
EY/EP/BG hotspots (misc)
CG5723	Ten-m	79E1	3L	10	10	33	10	0
CG5320	Gdh	95C13	3R	5	6	29	15	0
CG3979	Indy	75E1	3L	0	5	28	10	0
CG14450/  CG11367		80A1	3L	5	6	21	0	0
CG31522		82B4	3R	5	1	18	5	0
PA/PC hotspots
CG9216		14A9	X	0	5	4	0	50
CG14307		91A8	3R	0	3	4	0	40
Common hotspots
CG31243	cpo	90D1	3R	90	14	56	60	10
CG8276	bin3	42A14	2R	60	87	30	0	40
CG17161	grp	36A10	2L	45	25	20	45	50
CG12052	lola	47A11	2R	55	18	13	75	0
CG6889	tara	89B13	3R	25	13	34	50	30
CG8938	GstS1	53F9	2R	25	11	29	55	30
CG32529/  amn	amn	18F4	X	10	48	41	50	0
CG9755	pum	85C4	3R	35	19	20	40	30
CG3758	esg	35D1	2L	75	10	15	35	0
CG14709	BEST:CK0122	86E14	3R	30	19	55	30	0
CG8846	Thor	23F6	2L	10	44	24	40	0
CG3696	kis	21B6	2L	15	19	21	45	10
CG12284		72D1	3L	20	9	13	25	40
CG3903	Gli	35D4	2L	10	29	29	25	10
CG1856	ttk	100D1	3R	30	26	18	15	10
CG7437	mub	79A2	3L	35	6	23	35	0
CG31000	heph	100D4	3R	20	14	28	20	10
CG10645	lama	64D1	3L	20	8	29	25	10
CG9432	l(2)01289	42C7	2R	5	30	41	10	0
CG8651	trx	88B1	3R	15	18	25	15	10
CG5393	apt	59F1	2R	0	30	35	10	0
CG17950	HmgD	57F10	2R	20	20	26	5	0
CG17716	fas	50B6	2R	15	36	13	5	0
CG6072	sra	89B12	3R	0	16	26	5	20
CG7481/  CG7582	RhoGAP18B	18A3	X	0	24	24	15	0
CG3036		25B1	2L	0	25	16	20	0
CG5461	bun	33E5	2L	10	21	13	15	0
CG8804	wun	45D4	2R	5	21	9	20	0
CG10033	for	24A2	2L	0	26	23	5	0
CG2922	eIF-5C	83B1-2	3R	5	3	15	15	10
CG8815	Sin3A	49B6	2R	0	13	4	20	10

Mutation rates are in hits per 10,000 unique, localized insertions: i.e., percentages × 100. The numbers of chromosomes used in the calculations were as follows: BG, 2000; KG, 8000; EY, 8000; PA/PC, 1000. Complete hotspot data are available on request.