TABLE 2

Oligonucleotide primers used in this study

PrimerSequencea
msal FL5′-TTAGTCGAC(SalI) AATGGTTGTTTCCTCT-3′
5′-CAACCCGGG(SmaI) TCTAATTCCCATCCAT-3′
msal (Δ2-31)5′-TTAGTCGAC(SalI) AATGCCCCCAGGTTCTTTATC-3′
5′-CAACCCGGG(SmaI) TCTAATTCCCATCCAT-3′
msal (Δ2-102)5′-TTAGTCGAC(SalI) AATGTTTCAACAGTGGGGTCC-3′
5′-CAACCCGGG(SmaI) TCTAATTCCCATCCAT-3′
msal (Δ487-533)5′-TTAGTCGAC(SalI) AATGGTTGTTTCCTCT-3′
5′-AGCCCCGGG(SmaI) CTAGTGAAATGTTGAAAACTG-3′
msal (w)5′-CGTTGGCCAACTCGACCCAG-3′
msal (pFA6a-l,x)5′-GGGGATCCGTCGACCTGCAGCGTACGAATTCCCATCCATAGGTGG-3′
msal (pFA6a-2,y)5′-GTTTAAACGAGCTCGAATTCATCGATGTACATATTCTATAAAACAC-3′
msal (z)5′-GGTTCTTTACCTCACGAACC-3′
repl5′-ATGGATTCTGATCGTTGTTTAACAGACGAA-3′
5′-TTACCAATCACTGCAAAAACTCGAACCCAA-3′
  • a The top and bottom sequences indicate the sense and antisense primers, respectively. Restriction enzyme sites are underlined.