TABLE 1

S. pombe strains for meiotic DNA break determination

StrainGenotypea
GP535h pat1-114 ade6-M26 end1-458
GP813h+ swi5-39 pat1-114 ade6-M26 end1-458
GP2496h+ rad32::ura4+ pat1-114 ade6-M26 ura4-D18
GP3269h rad22::ura4+ rti1:: kanMX4 pat1-114 ade6-M26 ura4-D18 end1-458
GP3574h mus81::kanMX pat1-114 ade6-M26
GP3581h+ rhp51::his3+ pat1-114 ade6-M26
GP3607h dmc1::ura4+ pat1-114 ade6-M26 ura4-D18
GP3650h smt-0 mei4::ura4+ pat1-114 ade6-M26 ura4-D18
GP3652h smt-0 rad50::kanMX6 pat1-114 ade6-M26
GP4233h smt-0 rhp51::his3+ dmc1::ura4+ pat1-114 ade6-M26 his3-D1 ura4-D18
  • a Mutations other than commonly used auxotrophies and mating-type alleles are described in the following references: pat1-114 (Iino and Yamamoto 1985), end1-458 (Uemura and Yanagida 1984), rad32::ura4+ (Tavassoli et al. 1995), rad22::ura4+ (van den Bosch et al. 2001), rti1::kanMX4 (also known as rad22B::kanMX4; van den Bosch et al. 2002), mus81::kanMX (Boddy et al. 2001), rhp51::his3+ (Grishchuk and Kohli 2003), dmc1::ura4+ (Fukushima et al. 2000), mei4::ura4+ (Horie et al. 1998), rad50::kanMX6 (Hartsuiker et al. 2001), swi5-39 (Schmidt et al. 1987), and h smt-0 (Styrkarsdottir et al. 1993).