Screening for restoration of Cf-9 function

Progeny screenedNecrotic seedlingsaFunctional recombination intervalb (bp)
(Cf-9D508N/Cf4) × Avr95,4500150
(Cf-9R582Ter/Cf4) × Avr93,8940372
(Cf-9S675L/Cf4) × Avr96,3080654
  • a Necrotic seedlings arising due to intragenic recombination.

  • b Assuming recombination between Cf-9 and Cf-4 through tract III; Figure 1. The distance in base pairs between the point mutation in the corresponding mutant allele of Cf-9 and the sequence encoding the L457F Cf-9 specificity determinant is shown.