TABLE 2

Selection and molecular analysis of homozygous S2 recombinants

Genotype at MWa
S1 (survivor)5′3′Genotype at MW1Recombinant classbRecombination interval (bp)Location 5′ of ATG of Hcr9-9E
G640 Cf-4 Cf-9 Cf-4 IIb19013
G641 Cf-4 Cf-9 Cf-4 IIb19013
H822 Cf-4 Cf-9 Cf-4 IIa143546
H881 Cf-4 Cf-9 Cf-4 IIb75494
H888 Cf-4 Cf-9 Cf-4 IIa1442015
H889 Cf-4 Cf-9 Cf-4 IIb116361
H891 Cf-4 Cf-9 Cf-4 IIa492954
H896 Cf-4 Cf-9 Cf-4 IIa2113762
J091 Cf-4 Cf-9 Cf-4 IIa1653311
V514d Cf-4 Cf-9 Cf-9 I1241266c
V517d Cf-4 Cf-9 Cf-4 IIa502904
V512d Cf-4 Cf-9 Cf-4 IIa492954
V516d Cf-4 Cf-9 Cf-4 IIa213394
V408d Cf-4 Cf-9 Cf-4 IIb75494
  • a Based on CAPS analysis with MW5 and MW3; see Figure 1.

  • b Based on DNA gel blot analysis; see Figure 4A.

  • c 5′ of ATG of 9D.

  • d Reported in Parniske et al. (1997) and Thomas et al. (1997).