TABLE 4

cdc1-2(Ts) suppression and Smf1p requirement

Suppressionb
Cellular functionGenesacdc1-1cdc1-2Smf1p requiredc
Vacuolar protein sortingvps32, etc.++Yes
VPS4, VPS13 +NDND
Mn2+ homeostasis SMF1 ++ND
ATX2 +NDND
ccc1 ND
Protein trafficking bsd2 ++Yes
Ubiquitination UBP1 ++Yes
bro1, ubc4, doa4 ND+Yes
rsp5 +NDND
Unknown cos16/per1 +NDNo
Endocytosis end3 NDND

ND, not determined. +, growth; –, no growth.

  • a Genes are indicated in either lowercase (loss of function) or uppercase (high-copy plasmids), according to the allele tested.

  • b Ability to alleviate the 30° or 34° growth defect of cdc1-1(Ts) or cdc1-2(Ts) strains, respectively.

  • c Ability to alleviate the 34° growth defect of a cdc1-2(Ts) smf1Δ strain.