TABLE 3

Whole-cell Ca2+ and Mn2+ contents

StrainCa2+ pmol/OD600aMn2+ pmol/OD600b
FY70616.3 ± 33.341.3 ± 7.0
FY70 vps20 Δ ::LEU2 561.7 ± 50.9129.9 ± 27.0
FY70 vps32 Δ ::LEU2 602.4122.6 ± 18.0
FY70 vps18 Δ ::HIS3 ND188.3 ± 40.0
FY70 vps4 Δ ::URA3 ND145.5 ± 10.0
FY111332 ± 6638.9 ± 8.3
FY11 vps20 Δ ::LEU2 1154 ± 48166.4 ± 50.1

ND, not determined.

  • a Cellular Ca2+ content was measured with 45Ca2+ tracer and is expressed as the mean of three experiments (content of vps32Δ::LEU2 was measured once).

  • b Cellular Mn2+ content was measured by atomic absorption spectroscopy.