TABLE 2

Class E vps phenotypes

Growth
GenotypeComplex3 mm Mn2+a100 mm Lib200 mm Ca2+c40°dcdc1-1(Ts) suppressione
VPS ++++
vps23 Δ ESCRT-I+
vps28 Δ ESCRT-IND
vps37 Δ ESCRT-IND
vps22 Δ ESCRT-IIND
vsp25 Δ ESCRT-II+
vsp36 Δ ESCRT-II+
vps20 Δ ESCRT-IIIA+
vps32 Δ ESCRT-IIIA+
vps24 Δ ESCRT-IIIB ± +++
vps2 Δ ESCRT-IIIB ± ++ND
vps4 Δ AAA-ATPase ± +++
vps27 Δ Vps27/Hse1 ± +++
hse1 Δ Vps27/Hse1 ± ++ND

+, growth; ±, intermediate growth; –, no growth.

  • a Growth in the presence of 3 mm MnCl2.

  • b Growth in the presence of 100 mm LiCl.

  • c Growth in the presence of 200 mm CaCl2.

  • d Growth at 40°.

  • e Suppression of the cdc1-1(Ts) conditional (30°) growth defect.