TABLE 1

Effect of increasing the region upstream of the distal promoter on the transcriptional activity of the S. lebanonensis Adh gene

ConstructsRNase mappingRT-PCR
Δ-931.00 ± 0.3611.00 ± 1.301
Δ-8306.85 ± 1.2519.83 ± 3.572
Δ-126410.06 ± 2.76240.28 ± 6.574
Δ-235814.65 ± 2.17462.07 ± 10.456
Δ-643114.92 ± 3.01768.86 ± 12.497

Adh mRNA content relative to Δ-93 ± standard deviation is shown. RT-PCR, real-time quantitative RT-PCR.