TABLE 3

Synergy of apn1 and apn2 with mph1 with respect to forward mutation rates to canavanine resistance

StrainMutation rate × 107Relative rate
Wild type6.3 ± 0.8911
3.2 ± 0.442
mph139.9 ± 4.616.3
19.4 ± 2.226.1
apn16.9 ± 0.9911.1
apn1 mph1117 ± 12.4118.6
apn28.2 ± 1.111.3
apn2 mph132.4 ± 3.715.1
apn1 apn25.1 ± 0.9110.81
apn1 apn2 mph1212 ± 23.3133.6
apn1 rev33.6 ± 0.5321.1
apn1 rev3 mph13.4 ± 0.4821.1
  • Total cell numbers for calculation of mutation rates were determined from viable titer except for the values with the index 2, where cell number was determined from hematocytometer counts. Relative rates were calculated by normalizing mutant mutation rates to the respective wild-type rate in that particular experiment, which is indicated by the indices 1 and 2. The errors indicated are the quotients of the standard deviations of the number of mutations (Lea and Coulson 1948) and total cell numbers.