TABLE 2

Influence of defects in different repair pathways on the forward mutation rate of mph1 mutants to canavanine resistance

StrainMutation rate × 107Relative rateStrainMutation rate × 107Relative rate
Wild type2.9 ± 0.4211mph123.5 ± 2.718.1
1.6 ± 0.24212.4 ± 1.527.8
2.7 ± 0.40331.2 ± 3.6311.6
3.1 ± 0.61446.2 ± 5.3414.9
2.9 ± 0.43538.5 ± 4.2513.3
2.9 ± 0.46632.2 ± 3.1611.1
mag1a2.9 ± 0.4211.0yku701.6 ± 0.2530.59
mag1 mph1a41.1 ± 4.3114.2yku70 mph129.4 ± 3.3310.9
rad26a1.0 ± 0.2510.35yku802.3 ± 0.3840.74
rad26 mph1a27.3 ± 3.119.4yku80 mph135.0 ± 4.0411.3
rad28a0.7 ± 0.1120.44lig44.1 ± 0.5951.4
rad28 mph1a9.3 ± 1.125.8lig4 mph136.1 ± 4.1512.4
mgs14.9 ± 0.7761.7
mgs1 mph145.7 ± 5.5615.8
  • Total cell numbers for calculation of mutation rates were determined from viable titer. Mutation rates for wild type and mph1 were determined in each set of experiments. Relative rates were calculated by normalizing the mutant mutation rates to the wild-type rate determined in that particular experiment, which is indicated by the indices 1–6. The errors indicated are the quotients of the standard deviations of the number of mutations (Lea and Coulson 1948) and total cell numbers.

  • a For these strains, cell numbers were determined from hematocytometer counts.