TABLE 3

Effects of MPT5, SIR2, and SSD1-V on silencing

GenotypeTelomere
silencing
rDNA
silencing
mpt5Δ
sir2Δ
pADH_MPT5
pADH_MPT5 sir2Δ
ssd1Δ
SSD1-V
  • Stated effects on silencing are relative to silencing in the wild-type ssd1-d background. Telomere and rDNA silencing were measured by color formation using an ADE2 marker integrated into subtelomeric or ribosomal DNA. To detect decreased rDNA silencing relative to wild type in the sir2Δ strain, an rpd3Δ mutation was introduced into the wild-type background.