TABLE 1

Results of direct sequencing with universal ITS primers

Polyploid raceClean direct sequencesAdditive direct sequences
T1 (D1 × D3)D3: G1133, 1136, 1180,a 1274, 1288,a 1361, 1367, 1427, 1763a;
D1: G1392
T2 (D3 × D4)D4: G1134,a 1188,a 1286, 1393,a 1811, 1854
T3 (D3 × D5A)D3: G1359, 1394, 1930, 1766, 2059, 2098, 2099, 2100, 2539;
D5A: G1397
T4 (D3 × D5B)D3: G1304, 1929, 1348, 3137;D3 + D5B: G1469, 1747, 2476, 2557, 2468, 2470
D5B: G2437, 2469
T5 (D1 × G. clandestina)D1 + G. clandestina: G1487,a 1739,a 1969a
T6 (D1 × D5A)D5B: G1945
  • The first column lists accessions that had clean sequence (no significant secondary peaks on the electropherogram) and identifies which of the two parental homeologues resulted. The second column shows accessions that resulted in an additive signal from both homeologues (double peaks or unreadable sequence on the electropherogram).

  • a Sequences were presented in a previous publication (Rauscher et al. 2002).