TABLE 1

Bacterial plasmids and strains used in this study

Plasmid or strainDescriptionReference
Plasmid
    pFaOrfInverted terminal repeat sequences of Famar1, open
reading frame through one end
This study
    pFaLacTetpFaOrf containing lacZYA and TetR gene from pRZ1495This study
    pACFaLacTetFaLacTet Famar1 transposon cloned onto pACYC184This study
    pmmORFInverted terminal repeat sequences of Himar1 with
internal cloning sites
Lampe et al. (1999)
    pminiAMA mini-Ammar1 plasmidLampe et al. (2001)
    pACMarKanKanR Himar1 on p15a ori plasmidLampe et al. (1999)
    pBADC9Hyperactive version of Himar1 transposaseLampe et al. (1999)
    pRZ1495Tn5 papillation factor: the source of lacZYA-TetRMakris et al. (1988)
    pACYC184p15a ori cloning vectorChang and Cohen (1978)
    pCDNAIIColE1 ori cloning vectorInvitrogen (San Diego)
    pOJ427Source of the acc(3)IV (Apr R) gene cassetteB. Schoener (unpublished
results); see also Kieser et al. (2000)
    pEB17Apr R cassette from pOJ427 inserted as BamHI/XbaI blunt
fragment into pFaORF at BglII site
This study
    pEB18Apr R cassette from pOJ427 inserted as BamHI/XbaI blunt
fragment into pmmORF at BglII site
This study
    pEB19ITRs and Apr R from pEB17 inserted as BamHI fragment
into BamHI site of pACYC184; mating-out plasmid
for Famar1
This study
    pEB20ITRs and Apr R from pEB18 inserted as BamHI fragment
into BamHI site of pACYC184; mating-out plasmid for
Himar1
This study
    pBAD24E. coli expression vectorGuzman et al. (1995)
    pBADFa1-pBADFa40Coding sequences of Famar1 cloned into the NdeI/PstI
restriction sites of pBAD24
This study
    pBADAm1-pBADAm53Coding sequences of Ammar1 cloned into the NdeI/PstI
restriction sites of pBAD24
This study
    pDL129Ancestral Famar1 coding sequence in pBAD24This study
Strain
    DH5αendA1 hsdR17 (r- m+k k) supE44 thi-1 recA1 gyrA(NalR) relA1
Δ(lacIZYA-argF)U169 deoR80dlacΔ(lacZ)M15)
Hanahan (1983)
    RZ212Δ(lac-pro) ara str recA56 srl thi/ pOX38-GenJohnson and Reznikoff (1984)
    RZ221polA Δ(lac-pro) ara str nalJohnson et al. (1982)
    EB8RZ212/pOX38-Gen(F′)/pEB19; Famar1 mating-out strainThis study
    EB9RZ212/pOX38-Gen(F′)/pEB20; Himar1 mating-out strainThis study
    DL13DH5α F′::miniFamar1LacTet from pFaLacTet; Famar1
papillation strain
This study