TABLE 2

Oligonucleotides used in this study

OligonucleotideUse
GCCACTATCGACTACGCGATCASequencing insert in YCp50
CACGATGCGTCCGGCGTAGASequencing insert in YCp50
AACGATGAATTGCAAGGCGGTAACCATTAGTTTATTACTGTTGALG9 disruption forward
  TTTTAGTTTTGCTGGCCGCATC
CACCGTCGATGAATGCAGCACATGCCAGCTTATTCCAGTTATCALG9 disruption reverse
  CACAATGATGGGTAACAAGAGC
ATGCTGCTGACTACATCAAGCGATAAGGCGATCGCTAGTCAAZDS1 disruption forward
  AGGTTAGTTTTGCTGGCCGCATA
TATGTACGTGTGATGTGTATATGTCTATGTATGCAGCGCTGAAZDS1 disruption reverse
  GCCAATGATGGGTAACAAGAGC
TTATTTGTAACCTCCTATCTTTAGTTGAACTGATCCAAAAACAPRK1 disruption forward
  CGGATCCCCGGGTTAATTAA
CTTTTAATATTACATAGTCTATTATGTGTGAGAGCAAGTTGAAPRK1 disruption reverse
  TTCGAGCTCGTTTAAAC
TACTCTGCATAATTAGGTATTTTAAGCAACCAGATAAATCAACARK1 disruption forward
  CGGATCCCCGGGTTAATTAA
CCTCTTCAGAGATCGATCCGGTTCTGTTGAGCCAAATACGAATARK1 disruption reverse
  TCGAGCTCGTTTAAAC
CAGTCACTTCCATAAAGTTGGTACTGTTGGGTGAGGCAGCCGVPS21 disruption forward
  GATCCCCGGGTTAATTAA
CCCCTCTCTAACAACTGCAAGCACTGTTTGCGCTGGTCCCGAAVPS21 disruption reverse
  TTCGAGCTCGTTTAAAC
GGTTTAACTTATTTGGAGTAAACGTATATATTATTAACAGCGYPT52 disruption forward
  GATCCCCGGGTTAATTAA
GCAACTTCTGTTGTTTTTCCTCTAAAACACAAATCATAGGGAAYPT52 disruption reverse
  TTCGAGCTCGTTTAAAC
CAGCATCGAAGTTAAGTAGAATGGATAAACATACAGCAGCCGYPT53 disruption forward
  GATCCCCGGGTTAATTAA
CGCTAAGACGCTGAATATGCCGCGTTTGCTGTTGTTTTGCGAAYPT53 disruption reverse
  TTCGAGCTGCTTTAAAC
CAACCGATACTGATACATAGTAATAGAGGCAAACATAGAACGMutagenesis of SIW14: C214S forward
CGTTCTATGTTTGCCTCTATTACTATGTATCAGTATCGGTTGMutagenesis of SIW14: C214S reverse
CTGATACATTGTAATAGAGCCAAACATAGAACGGGGTGTTTGMutagenesis of SIW14: G217A forward
CAAACACCCCGTTCTATGTTTGGCTCTATTACAATGTATCAGMutagenesis of SIW14: G217A reverse
GTAATAGAGGCAAACATAAAACGGGGTGTTTGATTGGTTGMutagenesis of SIW14: R220K forward
CAACCAATCAAACACCCCGTTGTATGTTTGCCTCTATTACMutagenesis of SIW14: R220K reverse
GCGAAATGTTGGCAGAATGGCloning sklt2siw9 5′ region forward
CCATACGTCAATCGCCTTGGCloning sklt2siw 5′ region reverse
TTATTCGGAGAATCCTGTCGCloning sklt2siw 3′ region forward
CGAGCTACAACAAGAGCACGCloning sklt2siw 3′ region reverse