TABLE 1

The effect of MSS4 overexpression on the distribution of GFP-Spo14p

Class AaClass BClass CClass D
GFP-Spo14 + vector07.50.592
GFP-Spo14 + MSS4544942
GFP-Spo14K533E + vector03.5096.5
GFP-Spo14K533E + MSS4100.598.5

Strain AN123-4A was transformed with the indicated plasmids and examined in the fluorescence microscope and cells were grouped into classes on the basis of the observed distribution of GFP-Spo14p. The percentage of cells in each class is given. At least 400 cells were scored in each culture.

  • a Representative cells for each class are shown in Figure 4.