TABLE 2

REP3 plasmid stability assays

StrainGenotypeLoss rate (% per generation)
YSH316Wild type2.47 ± 0.69
YSH323 Δ sir2 1.83 ± 0.94
YSH496 Δ sir3 1.56 ± 0.73
YSH312 Δ sir4 3.09 ± 0.44
YSH480 GAL-SIR3 3.33 ± 1.07
YSH479 GAL-SIR4 2.96 ± 0.29
YSH474Wild type0.59 ± 1.08
YSH482 Δ mlp1 0.87 ± 0.88
YSH512 Δ mlp2 0.91 ± 0.35
YSH508Δmlp1 Δmlp22.16 ± 1.11
YSH565 Δ yku70 0.63 ± 0.44
YSH566 Δ yku80 1.20 ± 0.33
YSH564 Δ esc1 1.77 ± 0.63

The loss rates of YEp51 or YEp53 were measured in the indicated strains; a partial genotype is shown. YEp51 and YEp53 depend on REP3 for segregation function. Plasmid stability assays with YSH480 and YSH479 were carried out in raffinose/galactose media, causing overexpression of SIR3 or SIR4, respectively.