TABLE 1

Two-hybrid interactions between Fus1p and Sho1p and Bni1p

Fus1p-SH3(401–513)Fus1p-SH3(W473S)Fus1p(P422A)-SH3
Vector0.1 ± 0.010.01 ± 0.0010.05 ± 0.06
Sho1p-SH3302 ± 32114 ± 330.07 ± 0.01
Sho1p-SH3(P352A)0.02 ± 0.010.03 ± 0.0020.1 ± 0.2
Bni1p122 ± 133 ± 0.4119 ± 10

Assays were done as described (see materials and methods); the plasmids used were p2100[Fus1p-SH3(401–513)], p3475[Fus1p-SH3(W473S)], p3479[Fus1p(P422A)-SH3], p3505(Sho1p-SH3), p3492[Sho1p-SH3(P352A)], and p717[Bni1p (1–1214)]; pJG4-5 was the vector control. Two-hybrid reporter lexAop-lacZ expression was measured as β-galactosidase activity (in Miller units), and a mean and standard deviation were calculated from three independent samples.