TABLE 1

Primer sequences

GenePrimers, 5′–3′, forward first
MFA1 (YDR461w): α-pheromone, chromosome 4MFL-5px: CTG TTG CTC GGA TAA AAT CAA G
MFL-6px: GGA TAA CAG TAA CAG CGC TAA G
MFα1 (YPL187w): α-pheromone, chromosome 16sMFG1-U: AAA GCA ACA ACA GGT TTT GG
sMFG1-L: CAA ATT GAA ATA TGG CAG GC
MFAL-SEQF*: TTT TAA TAC ACA CAA ATA AAT TAT CC
MFAL-SEQR*: TGA GAA AGT TGA TTT TGT TAC GC
STE2 (YFL026w): α-pheromone receptor, chromosome 6STE2-142F: ACT GTT ACT CAG GCT ATT ATG TTC G
STE2-1539R: TAA TCC AAT GAA AAA AAA TCA CTG C
STE2-497F*: TGA CAT CAA TAT CTT TCA CTT TCA CTT TAG G
STE2-1078F*: TCA GAA AGA ACT TTT GTT GCT GAG G
STE2-1148R*: CCT TGT ATT TTT TGA ACT CGT GG
STE2-235R*: AAA CTT GGT TGA TAA TGA AAA TTG G
STE3 (YKL178c): α-pheromone receptor, chromosome 11STE3-F3: TGG ACA CAT TCA TTA CCT ACC ACG
STE3-ENDR: TTT CTG AAC TAA GCT CAT TTG AAC
STE3-530R*: GAA AAC GAA CAG CAC CAA GG
STE3-989F*: AGG ATT TAC AGC AGG TGG ATG G
STE3-997R*: TTT CAG AAT CGG TAG AGA ATG G
AGA2 (YGL032c): α-agglutinin subunit, chromosome 7AGA2-7PX: CTT TTG TTG TTC GGG CAT TTC C
AGA2-8PX: GTT GGC TAT TAT GAT AGT CCA TCC
SAG1 (YJR004c): α-agglutinin, chromosome 10SAG1-78F: GCT ATG TGA ACC AAA AAA AGA TAC C
SAG1-2005R: GCC TGA TGT TGA AGA ATA ATA TGC
SAG1-411R*: GTT TTT TGC GAT GAA TCT GAC AGC
SAG1-711F*: AAT GTC TGA TGT GGT GAA TTT CG
SAG1-1317F*: GTC GGA AGT AAT CAG TCA TGT GG
SAG1-1503R*: GAT GTT GAA GTC ACA ATA GGT ACG
  • * Internal sequencing primers.