Data summary for missense suppressors of Kek1-GFP misexpression in the eye and ovary

GMR-GAL4, UAS-kek1-gfpChangeEye (stock)Chorion (CY2-GAL4)GFP localization
Class I kek1-gfp ControlR3V3Apical
kek196-gfp L136F (CTC-TTC)R1V1–V2Apical
kek17C-gfp G160D (GGC-GAG)wtwtApical
kek153B-gfp G160S (GGC-AGC)wtwtApical
kek182-gfp G160S (GGC-AGC)wtwtApical
Class II kek1137-gfp P187S (CCC-TCC)R2V1Mislocalized
kek165-gfp P309L (CCC-CTC)R2V2Mislocalized
kek1118-gfp P309S (CCC-TCC)R2V1–V2Mislocalized
kek182A-gfp P329S (CCG-TCG)wtwtMislocalized
kek1176V-gfp P356S (CCA-TCA)R1V1–V2Mislocalized

Suppressors separated according to classes. Amino acid changes accounting for the suppression phenotype are shown. Suppressor stocks were maintained as recombinants with the GMR-GAL4 driver (stock), which were crossed to CY2-GAL4 to test suppression in the ovary. Eye phenotypes are classified as a range between mild roughness (R1) and severe roughness with a decrease in eye size (R4). Likewise, chorion phenotypes are classified according to the degree of ventralization, ranging from weak (V1) to complete ventralization (V4). GFP localization was followed in somatic follicle cells. Normal apical localization is observed with the control Kek1-GFP protein and class I suppressors, whereas class II suppressors display abnormal cellular distribution (mislocalized).