TABLE 2

Anonymous locus polymorphisms in the GMBX mapping panel

Locusa,bTypecPrimersAlleles detecteddSize rangee (bp)Informative meioses
FemalesMalesCombined
Mdo1L001 (AY369179)M-(CT)7F: 5′-ACTCTTTGTCTCTGAGTCT-3′2~176–178134234368
R: 5′-GCAGTTCCTGCTTAATTTG-3′
Mdo1L002 (AY369173)M-(TA)4(TG)17(TA)2F: 5′-GAAGGAAATTAAGAAACAAGTG-3′5127–141263218481
R: 5′-GATGTTTGATAAGTCCTATGTT-3′
Mdo1L003 (AY369180)M-(TA)3(CA)8F: 5′-GTCCTCCACCCAATAACCTT-3′6172–182192196388
R: 5′-ACTATGTGCTAGGCTCTCAG-3′
Mdo1L004 (AY369181)M-(CA)21F: 5′-ATACGAATTGGAAGACTGCA-3′8149–171257281538
R: 5′-TTCCTGGAAGAACTTCTTGA-3′
Mdo2L005 (AY369174)M-(TG)12F: 5′-ATTCTTCACACAGGAGGACA-3′8131–167288272560
R: 5′-GAAGGTAGTACCAAATGGAG-3′
Mdo2L006 (AY369175)M-(GT)16F: 5′-CATTGGCAACCTGTCCTCCTG-3′7196–220175266441
R: 5′-GCACTTAGCACAGTGTCTGGC-3′
Mdo3L007 (AY369178)M-(GT)11(GA)19F: 5′-TGCAGTTTGAGTCTTTCTGG-3′4f~135–272250297547
R: 5′-CCAGCCTATCTTAGATGTAG-3′
Mdo3L008 (AY369182)M-(GA)25F: 5′-ATCATTACTCACAATGAGCT-3′6171–183244313557
R: 5′-GAAGCTTCAATGAACAAT-3′
Mdo4L009 (AY369183)M-(GT)27F: 5′-AGAGAGCTGATTCTGAAGTA-3′8175–201206313519
R: 5′-CACAAATACTTTCAGGAGGA-3′
Mdo5L010 (AY369176)M-(CA)4(TG)2(CA)7F: 5′-ATCTAGGAAGCAAACCCAGGTCT-3′5176–200220217437
R: 5′-CTTGAGTCTATTACAAAGCCGTG-3′
Mdo5L011 (AY369184)M-(CA)13F: 5′-GTAGGTGGTGGATTCCTGCT-3′4262–27215250202
R: 5′-TTACTGCCTGCAGCCTGTAG-3′
Mdo5L012 (AY369185)M-(GA)18F: 5′-CAGAAATTTGCATACAATCA-3′11f~227–265239266505
R: 5′-GATCATAAGATATGGAAATA-3′
Mdo7L013 (AY369177)M-(CA)5(CG)2(CA)10F: 5′-TATCTTTGTAGACAGTAGTGAC-3′2127–129219150369
R: 5′-GATTGAATGGTTATGGATGTA-3′
Mdo8L014 (AY369186)M-(CA)25F: 5′-TGTCGGCTATGGGAAGGATG-3′10f181–219249200449
R: 5′-CTTCTGCCTTGGAACCAGAG-3′
Mdo8L015 (AY369187)M-(GT)19F: 5′-ATTCAGTCCAAGAACCTTG-3′7111–145221282503
R: 5′-TCCTCTGAAAGCGTAGCATA-3′
Mdo8L016 (AY369188)M-(GAGACA)2(GA)19F: 5′-TACTTTGGACAAGTCACCTA-3′6218–240254283537
R: 5′-TCCCATATAGTCTTGAACCT-3′
MdoXL017 (AY369189)M-(GT)19F: 5′-GATCTCATGGACTTAACTG-3′6119–139291(X-linked)291
R: 5′-TCTATTAGAATGCCCAGGA-3′
Mdo1L019 (AY369190)M-(CA)12F: 5′-CATGTGTCCCCACAGTCTTT-3′5121–133211305516
R: 5′-TGCTACTCACTTCTATTGAGTC-3′
Mdo1L020 (AY369191)M-(CT)32(CA)25F: 5′-TGTCCAGGGTCAAACATTGTA-3′5f195–229104160264
R: 5′-ACCAGATCTGAAGTCTTAGAAGG-3′
Mdo1L021 (AY369192)M-(GT)12F: 5′-GGGTAATCCACAAGTGTCCAA-3′9227–255234151385
R: 5′-TCCTCCTGAGATTGCCATTG-3′
Mdo2L022 (AY369193)M-(GT)12F: 5′-TTGGCTCTCTGCTTTCCAAT-3′7161–181269323592
R: 5′-AGCACATGCAGTCCTTTGAA-3′
Mdo2L023 (AY369194)M-(GT)14F: 5′-CCTCTGTGCTTTTCGGCTAT-3′6218–234285328613
R: 5′-ACTGGCATGGAATGGGACTA-3′
Mdo3L024 (AY369195)M-(GA)29F: 5′-TTTGTAGGGAGGTAGAAGATAGG-3′7173–215248274522
R: 5′-TGTGTTCAATGTGGGTCTCAA-3′
Mdo3L025 (AY369196)M-(GT)12(GA)24F: 5′-CCTTCAGGGTATAGTATGTTC-3′11254–286292331623
R: 5′-TGAATGGCTGCTTTACTGAA-3′
Mdo3L026 (AY369197)M-(GT)17G(GT)6F: 5′-TTATGGGATCTGGAATTTGAGG-3′5113–149261283544
R: 5′-TTAAATCTCTGGCAACCACA-3′
Mdo4L027 (AY369198)M-(CA)12F: 5′-GAAGGTAATACATCACATGACCACA-3′589–103245246491
R: 5′-TCCTCTACAATAGGAACCTCTTATC-3′
Mdo4L028 (AY369199)M-(CA)15F: 5′-TTTCTGCATTGTCCATGTCC-3′9f135–153272330602
R: 5′-CACATTGCCCATTTCCTGAT-3′
Mdo4L029 (AY369200)M-(CA)27F: 5′-TGGAATACCACCTATTCTCTGC-3′7112–134285268553
R: 5′-TTGCCTCCTGTCACTTTGGA-3′
Mdo5L030 (AY369201)M-(GT)26(GC)5F: 5′-TGACTCAGACTTAACCCCATCC-3′4143–149188226414
R: 5′-CCGAAGGGATCTCAAGTTCA-3′
Mdo6L031 (AY369202)M-(CT)2(GT)14F: 5′-ACGGTATGTTCTCTGACCGTCT-3′9f120–158247285532
R: 5′-TGTGCAGGACTACAGGGCAA-3′
Mdo6L032 (AY369203)M-(CA)13F: 5′-CATTCTTCATTTCATGACCAC-3′492–108199296495
R: 5′-GTACTGGGCAACAGTATATTGAG-3′
Mdo7L033 (AY369204)M-(CA)20F: 5′-CCTTGGGCATGATCAAGTTT-3′4f158–162213267480
R: 5′-TGCATGACCAAACACCTAA-3′
Mdo8L034 (AY369205)M-(CA)17F: 5′-TCCCATTTCTCTTCCACTCTT-3′6105–132287332619
R: 5′-GGAGGTGATTGGTTCACAATG-3′
Mdo8L035 (AY369206)M-(GT)7GG(GT)20F: 5′-GAAAATCTGCCCTTCCATGA-3′8108–154261281542
R: 5′-TCATCAAATGAGGCTCAAAG-3′
Mdo1LR01 R-SVOPA-15 (5′-TTCCGAACCG3′)g4h600–80088146234
Mdo1LR02 R-PAOPB-16 (5′-TTTGCCCGGA-3′)2 (P, A)82039166205
OPB-17 (5′-AGGGAACGAG-3′)
Mdo1LR03 R-PAOPA-15 (5′-TTCCGAACCC-3′)2 (P, A)3803377110
OPA-16 (5′-AGCCAGCGAA-3′)
Mdo1LR04 R-PAOPB-14 (5′-TCCGCTCTGG-3′)2 (P, A)>1500i65111176
Mdo1LR05 R-PAOPA-11 (5′-CAATCGCCGT-3′)2 (P, A)61056763
Mdo2LR06 R-PAOPA-10 (5′-GTGATCGCAG-3′)2 (P, A)90044103147
Mdo3LR07 R-PAOPA-10 (5′-GTGATCGCAG-3′)2 (P, A)830256085
Mdo3LR08 R-PAOPA-14 (5′-TCTGTGCTGG-3′)2 (P, A)7205283135
Mdo3LR09 R-PAOPB-08 (5′-GTCCACACGG-3′)2 (P, A)>2000i5260112
Mdo3LR10 R-PAOPB-11 (5′-GTAGACCCGT-3′)2 (P, A)>1500i503686
Mdo3LR11 R-PAOPB-11 (5′-GTAGACCCGT-3′)2 (P, A)405110165275
Mdo4LR12 R-PAOPA-06 (5′-GGTCCCTGAC-3′)2 (P, A)1350136100236
OPA-18 (5′-AGGTGACCGT-3′)
Mdo4LR13 R-PAOPA-11 (5′-CAATCGCCGT-3′)2 (P, A)100074105179
OPA-12 (5′-TCGGCGATAG-3′)
Mdo4LR14 R-RFLPOPB-05 (5′-TGCGCCCTTC-3′)2MboI fragmentsj4670116
OPB-08 (5′-GTCCACACGG-3′)
Mdo4LR15 R-PAOPB-06 (5′-TGCTCTGCCC-3′)2 (P, A)550127158285
Mdo4LR16 R-PAOPB-08 (5′-GTCCACACGG-3′)2 (P, A)620115197312
Mdo5LR17 R-PAOPA-01 (5′-CAGGCCCTTC-3′)2 (P, A)100020929
Mdo6LR18 R-PAOPA-02 (5′-TGCCGAGCTG-3′)2 (P, A)7107481155
Mdo6LR19 R-PAOPA-06 (5′-GGTCCCTGAC-3′)2 (P, A)1450113198311
OPA-18 (5′-AGGTGACCGT-3′)
Mdo6LR20 R-PAOPB-08 (5′-GTCCACACGG-3′)2 (P, A)<2000i114174288
Mdo6LR21 R-PAOPB-14 (5′-TCCGCTCTGG-3′)2 (P, A)>1500i6488152
Mdo7LR22 R-PAOPA-05 (5′-AGGGGTCTTG-3′)2 (P, A)1100312455
Mdo7LR23 R-PAOPA-09 (5′-GGGTAACGCC-3′)2 (P, A)9209180171
OPA-20 (5′-GTTGCGATCC-3′)
Mdo7LR24 R-PAOPB-16 (5′-TTTGCCCGGA-3′)2 (P, A)1700115198313
OPB-17 (5′-AGGGAACGAG-3′)
Mdo8LR25 R-RFLPOPB-02 (5′-TGATCCCTGG-3′)2RsaI fragmentsk10986195
MdXLR26 R-PAOPA-15 (5′-TTCCGAACCC-3′)2 (P, A)31084(X-linked)84
OPA-16 (5′-AGCCAGCGAA-3′)
Mdo7LR27 R-PAOPB-08 (5′-GTCCACACGG-3′)2 (P, A)9205451105
Mdo8LR28 R-PAOPB-11 (5′-GTAGACCCGT-3′)2 (P, A)10004658104
  • a Temporary anonymous locus nomenclature. Example: Mdo1L003: Mdo, Monodelphis domestica; 1L, linkage group 1 identifier; 003, unique numeric locus identifier. Example: Mdo7LR27: linkage group 7, RAPD locus 27. Linkage group identifiers are temporary; they will be replaced by chromosome identifiers when loci are physically mapped. Unique numeric locus identifiers are permanent.

  • b GenBank accession number is in parentheses.

  • c M- indicates microsatellite marker, followed by its major repeat motif. R- indicates RAPD marker. R-SV, RAPD-fragment size variation; R-PA, RAPD-presence/absence variation; R-RFLP, RAPD-restriction fragment length variation. See text for details.

  • d In GMBX panel only; all loci except Mdo1L001 and Mdo1L019 exhibited additional alleles in animals derived from other populations.

  • e Except where indicated by ~, sizes were determined by comparison to a corresponding M. domestica cloned microsatellite standard. Sizes preceded by ~ were estimated from comparison with commercial DNA ladder standards.

  • f Includes null (nonamplifying) allele(s).

  • g Primer oligonucleotide codes are those of Operon Technologies (Alameda, CA) as supplied with their RAPD 10-Mer kits A and B.

  • h Mdo1LR01 had four detectable alleles: A (800 bp), B (780 bp), and C (600 bp) were codominant; the fourth allele, b, had identical mobility to allele B (780 bp), but had very low fluorescence, rendering it recessive to allele B.

  • i Base-pair sizes preceded by > or < indicate band positions that are, respectively, slightly larger than or smaller than the nominal size indicated.

  • j Two alleles; A, 515-bp fragment; B, 435-bp fragment + (assumed 80-bp fragment; not detected).

  • k Two alleles; A, 440-bp fragment; B, 290- + 150-bp fragments.