TABLE 5

Response of mutants to serotonin agonists

5-HTNSD
A. Response to 7.5 mm serotonin (5-HT)
N25232.7
egl-30(md186sy676) 1.6201.5
egl-1 19.2158.7
egl-30(md186sy676); egl-1 12.5145.9
syls36 1.4121.1
α-MethylNSDddH2ONSD
B. Response to α-methyl 5-HT, a 5-HT2 receptor agonist
N215103.81.3151.2
egl-30(md186) 182NA8
egl-30(md186sy676)a 5.3152.55.4103.8
egl-1 1584.21.182.4
egl-30(md186sy676); egl-1a 19.3103.23.6102.4
C. Response of syls36 to α-methyl 5-HT
N219.5a,*88.26.585.1
syls36 34b,**142.40.4120.7

(A)Individual worms were assayed in M9 solution at room temperature. Eggs were counted after 90 min.

(B)Individual worms were assayed in a solution of 1 m α-methyl 5-HT in water at room temperature. Eggs were counted after 1 hr.

(C)Single worms were assayed in a total volume of 200 μl. Eggs were counted after a 90-min incubation at room temperature. Approximately 2 out of 10 animals from each genotype exploded in water over this longer time period, and these animals were not included in the assay. Two-tailed P values (Mann-Whitney U-test nonpaired comparisons of experimental to water controls) are indicated for each value due to high responses in water:

NA, not applicable; this strain explodes in ddH2O. SD, standard deviation per worm.

  • * P = 0.008, very significant

  • ** P < 0.0001, extremely significant.

  • a Hermaphrodites laid more eggs than wild type in water. The response to egl-30(md186sy676) mutants is not significant, and this is due to its high response in water.

  • b Two of 10 animals exploded when placed in water.