TABLE 2

Egg-laying response of 1-day-old adult hermaphrodites to pharmacological agonists

5-HTLevamisole + 5-HT
GenotypeResponseNSDResponseNSD
N25.0232.73.7150.6
egl-30(md186) 1.9102.60.5150.1
egl-8(md1971) 9.3124.1
egl-8(n488) 01900.3150
egl-8(sa47) 9.3104.4
unc-13(e51) 4.4113.7
egl-30(md186); syls105 5.5223.18.2150.3

Controls in M9 were performed on each strain. Only egl-30(md186); syls105, which contained the integrated egl-30::GFP transgene, responded by laying an average of 1.8 eggs per worm in M9. To measure response to 7.5 mm 5-HT, single worms were assayed in a total volume of 150 μl. Eggs were counted after a 90-min incubation at room temperature. SD, standard deviation per worm. To measure response to 7.5 mm 5-HT and 6.25 μm levamisole, five worms were assayed in a total volume of 150 μl. Eggs were counted after a 1-hr incubation at room temperature. SD, standard deviation per five worms.