The s.d. [ PIN+] variants destabilize weak [ PSI+] introduced by cytoduction

L2668 donorL2692 donor
Weak [PSI+]Unstable [PSI+]Weak [PSI+]Unstable [PSI+]
Recipient[ PIN+ ][pin][ PIN+ ][pin][ PIN+ ][pin ][PIN+][pin]
Low s.d.00300040
Medium s.d.05a5 + 5a00400
Very high s.d.08a8a004a4a0
High m.d.40005000

Numbers represent independent cytoductant colonies obtained from each donor and recipient pair. Recipients were 74-D694 derivatives with different [PIN+] variants. Donors contained stable weak [ PSI+]. L2668 is a derivative of L1845 rnq1Δ that was cytoduced with the L1767 original unstable [PSI+]. L2692 is a L1845 rnq1Δ derivative that was cytoduced with the N21 stable weak [PSI+] (Derkatch et al. 1996). Each cytoductant was scored for [ PSI+] stability (weak vs. unstable) and for the ability to form Rnq1-GFP dots ([ PIN+ ]or [pin]). The unnamed s.d. [PIN+] recipient in the first row is from the L1767 unstable [PSI+] derivative.

  • a These cytoductants gave rise to unstable [ PSI+] colonies containing [ PIN+], as well as weak [ PSI+] colonies lacking [ PIN+]. To reflect this, these cytoductants have been tabulated twice, once in the weak [ PSI+ ]/[pin] column and once in the unstable [PSI+]/[ PIN+] column. Note that the only derivatives (medium and very high) to lose [PIN+] in this manner were also the only derivatives that sometimes lost [ PIN+] during [ PSI+] induction (see Table 3).