TABLE 2

Fitness of ancestral and evolved strains and their combinations, relative to the plasmid-free ancestor

Ancestral host/ancestral plasmid (b0p0) or ancestral host (b0)Evolved host/evolved plasmid (b1p1)Evolved host (b1 or Embedded Image )Evolved host/ancestral plasmid (b1p0 or Embedded Image )Ancestral host/evolved plasmid (b0p1)
PopulationStrainFitness (±SE, no. of exp.)aStrainFitness (±SE, no. of exp.)StrainFitness (±SE, no. of exp.)StrainFitness (±SE, no. of exp.)StrainFitness (±SE, no. of exp.)
1CD1040.942 (±0.014, n = 26)CD1111.286 (±0.021, n = 15)CD1201.300 (±0.018, n = 17)CD1251.286 (±0.020, n = 13)CD1301.019 (±0.021, n = 15)
2CD1040.942bCD1121.303 (±0.016, n = 15)CD1211.306 (±0.020, n = 15)CD1261.281 (±0.020, n = 13)CD1310.835 (±0.042, n = 15)
3CD1040.942bCD1131.309 (±0.012, n = 15)CD1221.231c (±0.039, n = 19)CD1271.302c (±0.035, n = 13)CD1320.992 (±0.013, n = 15)
4CD1050.792 (±0.023, n = 20)CD1141.260 (±0.016, n = 18)CD1231.294 (±0.022, n = 12)CD1281.233 (±0.024, n = 12)CD1330.984 (±0.015, n = 15)
5CD1050.792dCD1151.167 (±0.020, n = 17)CD1241.190 (±0.015, n = 10)CD1291.200 (±0.017, n = 10)CD1340.954 (±0.017, n = 15)
6CD1050.792dCD1161.349 (±0.019, n = 18)NDeNDCD1350.982 (±0.028, n = 14)
7J53-11.000CD1171.236 (±0.011, n = 15)CD1481.234 (±0.017, n = 15)
CD1491.127 (±0.038, n = 14)

Fitnesses were measured as the fitness relative to the common competitor CD100. ± standard error. All fitness values were corrected for the effect of the T5-resistance marker in CD100.

  • a Standard error, number of experiments.

  • b Fitness measurement is the same as for population 1.

  • c See results for details.

  • d This fitness measurement is the same as for population 4.

  • e ND, not determined.