Comparison of selected karyotypes for maize
| Chromosome squashes | 3D fluorescence: Daweet al. (1992, 1994) | |||||
|---|---|---|---|---|---|---|
| Chromosome or SC rank | McClintocket al. (1981) | Longley (1939); Rhoades (1950) | 3D sections: Gillies (1973) | SC spreads | ||
| Gilles (1981) | This work | |||||
| 1 | 14.9 (1.27) | 14.8 (1.30) | 15.8 (1.30) | 14.2 (1.26) | 14.9 (1.24) | 14.8 (1.27) |
| 2 | 12.0 (1.20) | 12.1 (1.26) | 12.3 (1.68) | 12.4 (1.20) | 11.9 (1.15) | 11.9 (1.08) |
| 3 | 11.2 (2.03) | 11.7 (2.01) | 10.8 (2.06) | 11.1 (2.03) | 11.3 (2.06) | 11.2 (2.02) |
| 4 | 10.6 (1.57) | 10.6 (1.59) | 10.0 (1.39) | 10.8 (1.68) | 10.5 (1.62) | 10.7 (1.56) |
| 5 | 10.7 (1.16) | 10.7 (1.20) | 10.1 (1.08) | 10.9 (1.21) | 11.0 (1.13) | 10.8 (1.10) |
| 6 | 8.9 (3.14) | 8.4 (3.22) | 9.5 (3.41) | 9.2 (3.01) | 9.1 (2.75) | 8.9 (2.63) |
| 7 | 8.4 (2.58) | 8.3 (2.72) | 9.0 (2.38) | 8.5 (2.38) | 8.8 (2.67) | 8.7 (2.76) |
| 8 | 8.8 (3.29) | 8.3 (3.26) | 8.2 (2.83) | 8.8 (2.92) | 8.2 (2.99) | 8.5 (3.04) |
| 9 | 7.7 (1.82) | 8.1 (1.85) | 7.4 (1.69) | 8.0 (1.89) | 7.7 (2.09) | 7.7 (1.92) |
| 10 | 6.7 (2.83) | 6.9 (2.80) | 6.7 (2.31) | 6.2 (2.43) | 6.5 (2.54) | 6.8 (2.43) |
| Average total length of sets (μm) | — | 556 | — | 353 | 421 | 331 |
| No. of observations | — | 28 | 2 | 5 | 14 | 30 |
The karyotypes were prepared using a variety of techniques including light microscopy of pachytene chromosome squashes, deconvolution-based, three-dimensional fluorescence light microscopy of intact pachytene nuclei, three-dimensional electron microscopic reconstructions of pachytene nuclei from serial thin sections, or electron microscopy of SC spreads. In all cases, primary microsporocytes from maize inbred KYS were used. For each karyotype, the average relative length ofeach SC is presented first as a percentage of the total length of the SC set, followed by the average arm ratio in parentheses.