Genotyping requirements | ||||
---|---|---|---|---|
Design | Parental haplotyping | Identifying recombinants | Genotyping recombinants^{a} | Total |
BC | 2 M_{L} | 4PN | 2LPN M/100 | 2PN (2 + LM/100) + 2M_{L} |
F_{2} | 2 M_{L} | 2PN | 2LPN M/100 | 2PN (1 + LM/100) + 2M_{L} |
Half-sib | 3-13 M_{L} | 8PN | 4LPN M/100 | 2PN (4 + 2LM/100) + (3-13)M_{L} |
L, target interval in centimorgans; assuming small L, proportion of recombination across target interval, ̃L/100; M_{L}, number of internal markers; N, total population size; P, proportion of each tail taken for selective genotyping.
↵^{a} For complete genotyping, M = M_{L}; for HS genotyping, M = 4 or 5; for GS-HS genotyping, M = 3.