TABLE 3

Results of linkage tests between Aly loci and the self-incompatibility locus in family 98E-15

Genotypes at the loci tested
PlantSI groupAly13Aly3aAly10.1
Parent 97F-13/5Not tested1/13aaA
Parent 97F 15-3Not tested3/23AaA/B
  1I1/23AaA
  2IV3/13aaA
  3I1/23aaA
  4II13/23aaA
  5II13/23AaA
  6II13/23aaA
  7III1/3AaA/B
  8III1/3aaA/B
  9IV3/13aaA
10I1/23?AaA/B
11IV3/13aaA
12II13/23aaA/B
13III1/3AaA/B
14IV3/13AaA
15IV3/13AaA
  • Sequences of Aly3, Aly8, and Aly10.1 were amplified from genomic DNA of the parental and progeny plants, using primers specific for these sequence types (Aly3 primers 3F + 3R and Aly10.1 primers Ap8F + srk5R, respectively).

  • a The restriction enzyme BpuAI was used to distinguish the “A” and the “a” sequence types.