Results of linkage tests between Aly loci and the self-incompatibility locus in family 98E-15
| Genotypes at the loci tested | ||||
|---|---|---|---|---|
| Plant | SI group | Aly13 | Aly3a | Aly10.1 |
| Parent 97F-13/5 | Not tested | 1/13 | aa | A |
| Parent 97F 15-3 | Not tested | 3/23 | Aa | A/B |
| 1 | I | 1/23 | Aa | A |
| 2 | IV | 3/13 | aa | A |
| 3 | I | 1/23 | aa | A |
| 4 | II | 13/23 | aa | A |
| 5 | II | 13/23 | Aa | A |
| 6 | II | 13/23 | aa | A |
| 7 | III | 1/3 | Aa | A/B |
| 8 | III | 1/3 | aa | A/B |
| 9 | IV | 3/13 | aa | A |
| 10 | I | 1/23? | Aa | A/B |
| 11 | IV | 3/13 | aa | A |
| 12 | II | 13/23 | aa | A/B |
| 13 | III | 1/3 | Aa | A/B |
| 14 | IV | 3/13 | Aa | A |
| 15 | IV | 3/13 | Aa | A |
Sequences of Aly3, Aly8, and Aly10.1 were amplified from genomic DNA of the parental and progeny plants, using primers specific for these sequence types (Aly3 primers 3F + 3R and Aly10.1 primers Ap8F + srk5R, respectively).
↵a The restriction enzyme BpuAI was used to distinguish the “A” and the “a” sequence types.