TABLE 2

Effect of modifiers on Prat, Prat:bw and P[ry+; bw+] mRNA expression in 0- to 4-hr-old adult fly heads

Transgene
Prat:bwP[ry+; bw+]
ModifierPigment
effecta
RT-PCR PratbRT-PCR bwbPigment
effecta
RT-PCR PratbRT-PCR bwb
Mod-1-1ss2.1; 2.32; 2.2ss1.4; 32.1; 2.3
Mod-1-2ss1; 1.4; 1.81.5; 1.5ne2.3; 2.51.6; 3
Mod-1-3ss0.6; 0.8; 0.90.7; 0.8ws0.6; 0.90.2; 0.4
Mod-1-4ss1.6; 31.4; 1.5ss2; 41.4; 3
Mod-1-5ss0.1; 0.20.1; 0.2ss0.1; 0.20.2; 0.2
Mod-2-1ss0.3; 0.50.3; 0.5ss0.4; 0.40.1; 0.1
Mod-2-2ss0.5; 0.70.5; 0.6ss0.1; 0.10.1; 0.2
Mod-2-3ws0.3; 0.7c0.1; 0.4cne0.1; 0.3c0.2c; 0.4
Mod-2-4ss0.7; 1.30.1; 0.3ne0.9; 10.1; 0.1
Mod-2-5se1.3; 1.4; 1.55; 6.7we1.5; 1.71.5; 2
Mod-3-1se1.5; 1.51.8; 2ne1.5; 1.51; 1.1
Mod-3-2ws0.2; 0.30.9; 1.4ne0.2; 0.4; 0.60.3; 0.8
Mod-3-3ws0.6; 1.41.2; 1.4ws0.8; 1.1; 1.30.3; 0.3; 0.6
Mod-3-4ws0.2; 0.71; 1.3; 2.4ss2.5; 2.7; 2.80.7; 1.2
Mod-3-5ss0.1; 0.30.2; 0.3ne0.4; 0.50.1; 0.3
Mod-3-6ws0.1; 0.2c0.1c; 0.3ne0.2; 0.5c0.3; 0.5c
Mod-3-7ss1.3; 2.4; 2.70.7; 0.7ss3.1; 3.24.5; 5
Mod-3-8ss0.3; 0.40.7; 0.7ne0.1; 0.30.2; 0.4
Mod-3-9ws0.3; 0.5; 0.73; 5ne2; 2.9; 31.2; 1.4
Mod-3-10ws0.8; 1; 1.32; 2.7ne1.3; 1.3; 1.41; 1.2
Mod-3-11ss0.3; 0.40.8; 0.8ss1.6; 1.84.5; 5
Mod-3-12ws0.2; 0.30.2; 0.3ne0.8; 10.8; 1
Mod-Y-1ss1.6c; 1.91.2; 1.5css1.6; 1.8c3.2; 3.5c
  • ss, strong suppressor; ne, no effect; ws, weak suppressor; we, weak enhancer; se, strong enhancer.

  • a The effect of the modifiers on eye color was observed for both transgenes in 0- to 4-hr-old young adult fly heads.

  • b RT-PCR values are expressed as the ratio between the level of gene x mRNA with and without the modifier relative to the rp49 mRNA. Here, rp49 was coamplified with each mRNA and its level was used to normalize the yield of mRNA detected for gene x. Gene x is the endogenous Prat mRNA in two different genotypes or gene x is one of the bw mRNAs derived from the Prat:bw or bw+ transgene.

  • c Indicates the relative values obtained from the experiments shown in Figure 2.