TABLE 4

Suppression of sit4 pre- and sit4 ubc-induced synthetic defects by the presence of additional amino acids in the medium

Supplements addedGrowth
Ala+
Arg-
Asn++
Asp-
Cys-
Gln-
Glu-
Gly-
Ile+
Met-
Phe+
Pro-
Ser++
Thr+
Tyr-
Val-
Ino-
Met Arg Val-
Cys Gln Glu-
Cys Gln Pro Val Arg-
  • Amino acids were classified according to their capability to restore growth of sit4 pre and sit4 ubc mutants: ++, strong suppression as shown in Figure 10A; +, weak suppression as shown in Figure 10B; -, no suppression as shown in Figure 10C. Single amino acids and inositol were added at concentrations used in SC medium (120 μg/ml).