Suppression of sit4 pre- and sit4 ubc-induced synthetic defects by the presence of additional amino acids in the medium
| Supplements added | Growth |
|---|---|
| Ala | + |
| Arg | - |
| Asn | ++ |
| Asp | - |
| Cys | - |
| Gln | - |
| Glu | - |
| Gly | - |
| Ile | + |
| Met | - |
| Phe | + |
| Pro | - |
| Ser | ++ |
| Thr | + |
| Tyr | - |
| Val | - |
| Ino | - |
| Met Arg Val | - |
| Cys Gln Glu | - |
| Cys Gln Pro Val Arg | - |
Amino acids were classified according to their capability to restore growth of sit4 pre and sit4 ubc mutants: ++, strong suppression as shown in Figure 10A; +, weak suppression as shown in Figure 10B; -, no suppression as shown in Figure 10C. Single amino acids and inositol were added at concentrations used in SC medium (120 μg/ml).