TABLE 2

Gene conversion and crossing over in wild-type and mms4 SK1 diploids

% gene conversion (no. asci)
GenotypeHIS4LEU2CENIIIMATLYS5MET13CYH2TRP5CENVIIITHR1CUP1
MMS4 1.4 (425)3.5 (425)0.5 (426)1.2 (424)2.3 (425)3.5 (425)0.9 (425)0.7 (425)0.7 (425)6.3 (425)11.3 (415)
mms4 8.8* (272)5.9 (271)0 (271)5.9* (268)8.5* (271)9.2* (271)4.4* (270)7.0* (272)3.1 (252)10.0 (270)12.6 (270)
Map distance (cM) (no. asci)
Chromosome IIIChromosome VIIChromosome VIII
GenotypeHIS4-LEU2LEU2-CENCEN-MATLYS5-MET13MET13-CYH2CYH2-TRP5CEN-TRP1TRP1-CUP1
MMS4 12.5 (404)10.5 (408)15.3 (419)18.7 (407)10.1 (407)41.2 (420)21.2 (398)23.5 (343)
mms4 8.4* (237)6.1* (254)10.7* (252)16.4 (229)8.5 (236)37.2 (240)13.9* (220)21.4 (217)

Isogenic SK1 diploids were sporulated at 30° and tetrads dissected (NHY957, wild type; NH455, mms4/mms4). Gene conversion was scored as tetrads that exhibited 3+:1 or 1+:3 segregation for the marker. Map distances were calculated using the formula from Perkins (1947).

  • * indicates the percentage is statistically significantly different from wild type at the 95% confidence level. Statistical analyses were performed using programs from the VassarStats website (http://faculty.vassar.edu/lowry/VassarStats.html) and the Stahl lab website (http://groik.com/stahl/).