TABLE 5

Phenotypes of single and double mutants

New mutationSingle mutantDouble mutant with class ADouble mutant with class B
lin-8(n111)lin-15(n767)lin-36(n766)lin-15(n744)
Class A lin-56(n2728) WTWTWTMuvMuv
Class B lin-13(n770) WTMuvMuvWTWT
lin-52(n771) WTMuvMuvWTWT
lin-53(n833) WTMuvMuvWTWT
lin-54(n2231) WTMuvMuvWTWT
dpl-1(n2994) WTMuvMuvWTWT

Mutations in new genes were separated from the original mutation present in the synMuv strain as described in materials and methods. Double mutants carrying mutations in these new genes and mutations in previously known class A or class B genes were constructed as described in materials and methods. WT, animals had wild-type vulval morphology as observed using a dissecting microscope, i.e., 0% Muv (n > 500). Muv, animals had a Muv phenotype of >50% penetrance (n ≥ 100). lin-13, lin-52, and lin-54 animals displayed a weaker Muv phenotype in double mutants with lin-8(n111) than in those with lin-15(n767). dpl-1 animals displayed a weaker Muv phenotype in a lin-15(n433) background than in a lin-15(n767) background.