TABLE 3

Three- and four-factor crosses

GeneGenotype of heterozygotePhenotype of selected recombinantsGenotype of selected recombinants (with respect to unselected markers)
lin-13 lin-13 unc-32+ +/+ + unc-49 dpy-18; lin-15(n767) Unc-320/2 unc-49 dpy-18/+ +
Dpy7/7 lin-13 unc-32/+ +
Unc-490/1 lin-13 unc-32/+ +
+ + lin-13/unc-93 dpy-27 +; lin-15(n767) Unc30/30 lin-13/+
+ + lin-13/unc-93 dpy-17 +; lin-15(n767) Unc8/8 lin-13/+
Dpy0/3 lin-13/+
+ lin-13 +/dpy-17 + unc-32; lin-15(n767) Dpy1/8 lin-13/+
Unc5/10 lin-13/+
lin-52 + + unc-32 lin-52/lon-1 sma-3 + +; lin-15(n767) Lon1/1 lin-52 unc-32/+ +
Sma0/4 lin-52/+
Muv7/7 lon-1/+
+ + lin-52/sma-3 unc-32; lin-15(n767) Sma3/3 lin-52/+
Unc0/13 lin-52/+
+ lin-52 +/dpy-19 + unc-69; lin-15(n767) Dpy17/31 lin-52/+
Unc2/11 lin-52/+
+ lin-52 +/unc-16 + unc-49; lin-15(n767) Unc-160/2 lin-52/+
Unc-4913/16 lin-52/+
+ lin-52 +/unc-16 + unc-47; lin-15(n767) Unc-477/9 lin-52/+
lin-52 + unc-69/+ stP127 +; lin-15(n767) Muv3/12 stP127/+
sma-3 + lin-52 +/+ sqv-3 + unc-69; lin-15(n767) Sma9/9 sqv-3/+
Muv1/27 sqv-3/+
Unc14/16 lin-52/+
lin-53 dpy-5 lin-53 + +/+ + lin-11 unc-75; lin-15(n767) Dpy7/7 lin-11 unc-75/+ +
Muv0/12 lin-11 unc-75/+ +
Vul0/10 dpy-5 lin-53/+ +
Unc8/8 dpy-5 lin-53/+ +
+ lin-53 +/unc-29 + lin-11; lin-15(n767) Unc1/3 lin-53/+
Vul11/19 lin-53/+
lin-54 lin-8; + + lin-54/unc-22 unc-30 + Unc-2219/19 lin-54/+
Unc-300/15 lin-54/+
lin-8; + lin-54 +/unc-30 + dpy-4 Unc2/11 lin-54/+
Dpy21/22 lin-54/+
lin-54 + +/+ lev-1 unc-26; lin-15(n433) Lev0/25 lin-54/+
dpl-1 + dpl-1 +/dpy-10 + unc-53; lin-15(n433) Dpy6/9 dpl-1/+
Unc6/11 dpl-1/+
+ dpl-1 +/rol-6 + unc-4; lin-15(n433) Rol6/14 dpl-1/+
Unc9/14 dpl-1/+
dpl-1 + +/+ let-240 unc-4; lin-15(n433) Unc25/25 dpl-1/+
lin-56 + + lin-56/unc-85 dpy-10 +; lin-15(n744) Unc4/4 lin-56/+
Dpy0/6 lin-56/+
+ lin-56 +/dpy-10 + unc-53; lin-15(n744) Dpy5/10 lin-56/+
Unc6/11 lin-56/+
+ + lin-56/rol-6 unc-4 +; lin-15(n744) Rol16/16 lin-56/+
Unc0/9 lin-56/+

Three- and four-factor crosses were performed as described previously (Brenner 1974; Ferguson and Horvitz 1989).