TABLE 2

Sequences of lin-13 class B mutations

AlleleWild-type sequenceMutant sequenceSubstitution
n770 CAGTAGQ1988amber
n2238 CAATAAQ996ochre
n2981 TGCTACC814Y
n2984 GGAGAAG360E
n2985 CAATAAQ1717ochre
n2988 TGTTATC361Y

Wild-type and mutant codons are shown with the mutated nucleotide underlined. All mutations were GC-to-AT transitions as expected for EMS-induced mutations (Anderson 1995). Amino acid substitutions are shown as wild-type residue identity, residue number, and predicted mutant residue.