Frequencies of 3:1 segregation
Predicted % of undiagnosed false tetrads | % 3:1 segregationc | |||||
---|---|---|---|---|---|---|
Straina | Total tetradsb | CAN1 | URA3 | HOM3 | TRP2 | |
Chromosome V | ||||||
TID1 | 1409 | 0.8 | 0.8/0.7 | 0.3/0.2 | 0.6/0.5 | 1.4/1.3 |
tid1-pTID1 | 1222 | 0.4 | 1.1/1.0 | 0.6/0.5 | 0.6/0.6 | 1.6/1.5 |
TID1 combined | 2631 | 0.6 | 0.9/0.8 | 0.4/0.3 | 0.6/0.5 | 1.5/1.4 |
tid1 | 1118 | 2.9 | 2.4/1.8* | 1.4/0.8 | 1.1/0.8 | 3.2/2.8* |
Predicted % of undiagnosed false tetrads | % 3:1 segregationc | |||||
Straina | Total tetradsb | MAT | LEU2 | URA3 | ||
Chromosome III | ||||||
TID1 | 1250 | 3.1 | 1.3/0.2d | 6.4/6.2 | 1.8/1.5 | |
tid1 | 1320 | 1.7 | 1.8/1.2* | 6.4/6.2 | 1.5/1.3 | |
DMC1-YEpRAD54 | 1275 | 0.4 | 0.7/0.5 | 4.5/4.5 | 0.9/0.8 | |
dmc1Δ-YEpRAD54 | 1362 | 0.6 | 0.4/0.2 | 7.6/7.5* | 0.7/0.6 |
Postmeiotic segregation was not detected in this experiment. Numbers presented are sums of the frequencies of 3+:1- and 1+:3- tetrads.
↵a See footnotes a and b of Table 2 for names of strains used and marker locations.
↵b Number of tetrads dissected minus the number of tetrads that were diagnostically false. The predicted percentage of undiagnosed false tetrads was calculated as [(Nf2 + NN3) - (Nm3:1 + NN4:0)]/Total × 100 (see appendix).
↵c Percentages of observed frequencies/percentages corrected for the contribution of false tetrads (see appendix) are shown. *, the corrected value of 3:1 frequency for a mutant is significantly different from that of the wild-type control on the basis of Fisher’s exact test.
↵d The large correction factor required makes this value less reliable than other estimates (see text).