TABLE 2

Summary of genotypic tests for six isogenic strains constructed in this study

Specific alleles
of STE20 gene
mtDNA: ND2
(AluI digest)b
Nuclear genotype characterized by five different markers
StrainsDaAaURA5
(HindIII digest)
M13(GACA)4OPA17RP4CHEF
JEC20+a--JEC20bJEC20JEC20JEC20JEC20JEC20JEC20
JEC21-+-JEC21JEC21JEC21JEC21JEC21JEC21JEC21
CDC46-++CDC46cCDC46CDC46CDC46CDC46CDC46CDC46
JEC20-mtA-1+--CDC46JEC20JEC20JEC20JEC20JEC20JEC20
JEC20-mtA-2+--CDC46JEC20JEC20JEC20JEC20JEC20JEC20
JEC20-mtA-3+--CDC46JEC20JEC20JEC20JEC20JEC20JEC20
JEC21-mtA-1-+-CDC46JEC21JEC21JEC21JEC21JEC21JEC21
JEC21-mtA-2-+-CDC46JEC21JEC21JEC21JEC21JEC21JEC21
JEC21-mtA-3-+-CDC46JEC21JEC21JEC21JEC21JEC21JEC21
  • CHEF, contour-clamped homogeneous electric field.

  • a “+” indicates the presence of the allele identified by a specific PCR primer pair. “-” indicates the absence of such an allele.

  • b The genotype at the indicated locus/loci was identical to JEC20. Except at the mating-type locus, JEC20 and JEC21 have identical genotypes as shown before (Kwon-Chung et al. 1992) and by all our current genotyping methods.

  • c Genotype was identical to that of CDC46 at the indicated locus/genetic marker.