Bacterial strains and plasmids
| Strain no. | Relevant genotypea | Reference/derivation |
|---|---|---|
| Previous studies | ||
| AB1157 | rec+ | Bachmann (1987) |
| JC2926 | recA13 | Clark and Margulies (1965) |
| JC5519 | recB21 recC22 | Willetts and Clark (1969) |
| JC5547 | recA13 recB21 recC22 | Willetts and Clark (1969) |
| JC10287 | Δ(recA-srlR)304 | Czonka and Clark (1979) |
| JC12123 | recJ284::Tn10 | Lovett and Clark (1984) |
| STL2694 | ΔxonA300::cat | Viswanathan and Lovett (1998) |
| N3033b | lacZ98::Tn10 | CGSC no. 6660 |
| This study | ||
| JB1 | Δ(recC-ptr-recB-recD)3::kan | Deletion replacement in AB1157 |
| AM1 | Δ(recA-srlR)304 Δ(recC-ptr-recB-recD)3::kan | JC10287c × P1 JB1 |
| AM2 | Δ(recA-srlR)304 Δ(recC-ptr-recB-recD)4 | In-frame deletion (AM1 Δkan) |
| AM3 | ΔrecF20::cat | Deletion replacement in AB1157 |
| AM4 | Δ(recA-srlR)304 ΔrecF20::cat | JC10287c × P1 AM3 |
| AM5 | ΔrecF20::cat Δ(recC-ptr-recB-recD)3::kan | AM3 × P1 JB1 |
| AM6b | lacZ98::Tn10 tsx::cat | Deletion replacement in N3033 |
| AK132 | Δ(recA-srlR)304 Δ(recC-ptr-recB-recD)3::kan recJ284::Tn10 | AM1d × P1 JC12123 |
| AK133 | Δ(recA-srlR)304 Δ(recC-ptr-recB-recD)3::kan ΔxonA300::cat | AM1d × P1 STL2694 |
| Plasmid | Replicon/drug resistance/other genes | Reference/derivation |
| Previous studies | ||
| pBEU14 | R1(Ts)/bla/recA | Uhlin and Clark (1981) |
| pDWS2 | pBR322/bla/recC-ptr-recB-recD | Ponticelli et al. (1985) |
| pB1082CD | pBR322/bla/recC-ptr-recBK1082Q-recD | Wang et al. (2000) |
| pMY330 | p15A/cat/recC-ptr-recBD1080A-recD | Yu et al. (1998b) |
| pSA122 | p15A/cat/recC-ptr-recB | Amundsen et al. (2000) |
| pWSK29 | pSC101/bla/- | Wang and Kushner (1991) |
| pWSK2988 | pSC101/bla/addAB | Kooistra et al. (1993) |
| pHSG415 | pSC101(Ts)/bla cat kan | Hashimoto-Gotoh et al. (1981) |
| This study | ||
| pAMP1 | pSC101/bla/recC-ptr-recB-recD | pWSK29::recBCD(WT) |
| pAMP2 | pSC101/bla/ΔaddAB | pWSK2988 ΔEcoRI |
| pAMP3 | pSC101/bla/recC-ptr-recB | pWSK29::recBC |
| pAMP5 | pSC101/bla/recC-ptr-recB*-recD | pWSK29::recB*CD |
| pAMP7 | pSC101/bla/recC-ptr-recB1080-recD | pWSK29::recB1080CD |
| pAMP8 | pSC101/bla/recD | pWSK29::recD |
| pEAK1 | pSC101(Ts)/bla | pHSG415 ΔEcoRI-HindIII |
| pK134 | pSC101(Ts)/bla/recC-ptr-recB | pEAK1::recBC |
| pK135 | pSC101(Ts)/bla/recA, recC-ptr-recB | pEAK134::recA |
↵a Full genotype: F-λ-rac-thi-1 hisG4 Δ(gpt-proA)62 argE3 thr-1 leuB6 kdgK51 rfbD1 araC14 lacY1 galK2 xylA5 mtl-1 tsx-33 glnV44 rpsL31.
↵b Other mutations are λ-, IN(rrnD-rrnE)1.
↵c The JC10287 recA defect was complemented for the P1 transduction with the pBEU14 plasmid, which was cured once the construct was confirmed.
↵d AM1 recA and recBCD defects were complemented for the P1 transduction with the pK135 plasmid, which was cured once the construct was confirmed.