TABLE 1

Bacterial strains and plasmids

Strain no.Relevant genotypeaReference/derivation
Previous studies
AB1157rec+Bachmann (1987)
JC2926recA13Clark and Margulies (1965)
JC5519recB21 recC22Willetts and Clark (1969)
JC5547recA13 recB21 recC22Willetts and Clark (1969)
JC10287Δ(recA-srlR)304Czonka and Clark (1979)
JC12123recJ284::Tn10Lovett and Clark (1984)
STL2694ΔxonA300::catViswanathan and Lovett (1998)
N3033blacZ98::Tn10CGSC no. 6660
This study
JB1Δ(recC-ptr-recB-recD)3::kanDeletion replacement in AB1157
AM1Δ(recA-srlR)304 Δ(recC-ptr-recB-recD)3::kanJC10287c × P1 JB1
AM2Δ(recA-srlR)304 Δ(recC-ptr-recB-recD)4In-frame deletion (AM1 Δkan)
AM3ΔrecF20::catDeletion replacement in AB1157
AM4Δ(recA-srlR)304 ΔrecF20::catJC10287c × P1 AM3
AM5ΔrecF20::cat Δ(recC-ptr-recB-recD)3::kanAM3 × P1 JB1
AM6blacZ98::Tn10 tsx::catDeletion replacement in N3033
AK132Δ(recA-srlR)304 Δ(recC-ptr-recB-recD)3::kan recJ284::Tn10AM1d × P1 JC12123
AK133Δ(recA-srlR)304 Δ(recC-ptr-recB-recD)3::kan ΔxonA300::catAM1d × P1 STL2694
PlasmidReplicon/drug resistance/other genesReference/derivation
Previous studies
pBEU14R1(Ts)/bla/recAUhlin and Clark (1981)
pDWS2pBR322/bla/recC-ptr-recB-recDPonticelli et al. (1985)
pB1082CDpBR322/bla/recC-ptr-recBK1082Q-recDWang et al. (2000)
pMY330p15A/cat/recC-ptr-recBD1080A-recDYu et al. (1998b)
pSA122p15A/cat/recC-ptr-recBAmundsen et al. (2000)
pWSK29pSC101/bla/-Wang and Kushner (1991)
pWSK2988pSC101/bla/addABKooistra et al. (1993)
pHSG415pSC101(Ts)/bla cat kanHashimoto-Gotoh et al. (1981)
This study
pAMP1pSC101/bla/recC-ptr-recB-recDpWSK29::recBCD(WT)
pAMP2pSC101/blaaddABpWSK2988 ΔEcoRI
pAMP3pSC101/bla/recC-ptr-recBpWSK29::recBC
pAMP5pSC101/bla/recC-ptr-recB*-recDpWSK29::recB*CD
pAMP7pSC101/bla/recC-ptr-recB1080-recDpWSK29::recB1080CD
pAMP8pSC101/bla/recDpWSK29::recD
pEAK1pSC101(Ts)/blapHSG415 ΔEcoRI-HindIII
pK134pSC101(Ts)/bla/recC-ptr-recBpEAK1::recBC
pK135pSC101(Ts)/bla/recA, recC-ptr-recBpEAK134::recA
  • a Full genotype: F-λ-rac-thi-1 hisG4 Δ(gpt-proA)62 argE3 thr-1 leuB6 kdgK51 rfbD1 araC14 lacY1 galK2 xylA5 mtl-1 tsx-33 glnV44 rpsL31.

  • b Other mutations are λ-, IN(rrnD-rrnE)1.

  • c The JC10287 recA defect was complemented for the P1 transduction with the pBEU14 plasmid, which was cured once the construct was confirmed.

  • d AM1 recA and recBCD defects were complemented for the P1 transduction with the pK135 plasmid, which was cured once the construct was confirmed.