TABLE 1

P1-wr Ufo1 × P1-wr backcross progeny analysis for Ufo expression and P1-wr DNA methylation

Inbred
carrying
P1-wr
allele
Pericarp/cob
pigmentation
phenotype of
ear parent of
backcross
progeny
Phenotypic classification for Ufo expressers vs. nonexpressersCorrelation of Ufo expression phenotype and P1-wr DNA methylationa
BackcrossTotalExpressersbNonexpresserscChi-square
probabilityd
TotalExpresser
and
hypermethylated
Expresser
and
hypomethylated
Nonexpresser
and
hypermethylated
Nonexpresser
and
hypomethylated
1. (P1-wr Ufo1 × P1-wr)BC4W22Red/Red20571134P < 0.00147017300
2. (P1-wr Ufo1 × P1-wr)BC5W23Red/Red4918310.05 < P < 0.1025010150
3. (P1-wr Ufo1 × P1-wr)BC4W23Red/Red6226360.20 < P < 0.3024010140
4. (P1-wr Ufo1 × P1-wr)BC4W23Red/Red581543P < 0.0012305180
5. (P1-wr Ufo1 × P1-wr)BC4W23Red/Red7030400.20 < P < 0.3030012180
  • a DNA methylation state of the P1-wr gene was determined for a subset of individual seedlings from each backcross progeny as described in text. Progeny were assigned to one of four classes on the basis of their Ufo expression phenotype and DNA methylation state.

  • b Expressers, no. of plants with increased pigmentation in kernel pericarp, cob, husk, and/or tassel glumes. This class includes plants that produced variegated husks and/or variegated pericarp.

  • c Nonexpressers, no. of plants with standard P1-wr-specified pigmentation pattern of colorless pericarp and red cob.

  • d Chi-square tests were performed on the phenotypic class data to test for 1:1 segregation ratios of Ufo expresser and nonexpresser plants.