TABLE 5

Fitness of diploids constructed from the progenitor haploid and FLC-resistant haploids from experiments 1 and 2

MIC
FLC
No. of doublings ±SD (n = 3 replicates)
DiploidsaTag XbTag YcTag 5 (all P5)
O2 × O1/P525613.8 ± 0.413.8 ± 0.49.5 ± 0.5
O2 × O3/P525613.2 ± 0.213.3 ± 0.28.7 ± 0.6
O1 × P1/P5169.0 ± 0.9-7.2 ± 0.7
O2 × P1/P51610.3 ± 1.210.1 ± 1.17.2 ± 0.7
O3 × P1/P5169.4 ± 0.79.7 ± 0.57.8 ± 0.9
O1 × D2/P56411.2 ± 0.511.2 ± 0.57.9 ± 0.4
O1 × D3/P512812.5 ± 0.812.2 ± 0.68.6 ± 0.4
O1 × D4/P56410.9 ± 0.111.3 ± 0.18.0 ± 0.1
O2 × D2/P56411.4 ± 0.2-7.8 ± 0.4
O2 × D3/P512812.7 ± 0.312.3 ± 0.18.6 ± 0.1
O2 × D4/P512811.3 ± 0.311.1 ± 0.47.9 ± 0.4
O3 × D2/P512811.2 ± 0.211.0 ± 0.27.7 ± 0.3
O3 × D3/P512813.2 ± 0.113.2 ± 0.19.0 ± 0.2
O3 × D4/P512810.8 ± 1.210.6 ± 1.27.3 ± 1.1
  • Mean number of doublings for all strains in 0.5× YPD in the absence of FLC was 13.6 ± 0.2 (data not shown).

  • a Diploids are listed with MATa strain first and the MATα strain second. Progenitor diploid P5 after the slash (/) was the reference strain in each fitness assay.

  • b Fitness of each diploid carrying two tags (e.g., D2 × D3) was measured with both tags (e.g., tag 2 and tag 3).

  • c Minus (-) means that only one tag was present in the hybrid diploid.