TABLE 5

Genes whose deletion results in an altered alkali-soluble β-glucan phenotype

Gene nameORFβ-1,6-Glucanβ-1,3-Glucan
β-1,6-Glucan only affected
Elevated
    MAP1YLR244C++wt
    ANP1YEL036C+wt
    ERG4YGL012W+wt
    ERV14YGL054C+wt
    ERV41YML067C+wt
    KEX1YGL240W+wt
    FYV5YCL058C+wt
    BUD30YDL151C+wt
    BUD27YFL023W+wt
YGL007W+wt
Reduced
YKL037W--wt
    CNE1YAL058W-wt
    FKS1YLR342W-wt
    KRE11YGR166W-wt
    PEP3YLR148W(-)wt
β-1,3-Glucan only affected
Elevated
    THP1YOL072Wwt+
YNL213Cwt+
    BTS1YPL069Cwt(+)
    GPA2YER020Wwt(+)
    FYV7YLR068Cwt(+)
Reduced
    SEC66YBR171Wwt-
    ACC1YNR016Cwt(-)
    SYS1YJL004Cwt(-)
β-1,6-Glucan and β-1,3-glucan affected
Both elevated
    END3YNL084C+++++
    VRP1YLR337C+++++
    SAC7YDR389W++++
    LAS17YOR181W+++(+)
    VAC8YEL013W+++
    FYV1YDR024W+++
    IPK1YDR315C++(+)
    FPS1YLL043W++
    SAC1YKL212W++
    VID21YDR359C++
    EAF6YJR082C++
    CDC25YLR310C+(+)
    GLY1YEL046C+(+)
    SUR4YLR372W+(+)
    VPS61YDR136C+(+)
    UTH1YKR042W(+)+
    VPS27YNR006W(+)+
    VPS67YKR020W(+)+
    FYV12YOR183W(++)(++)
Both reduced
    SMI1YGR229C-----
    PIN4YBL051C----
    CSF1YLR087C--
    LAS21YJL062W--
    COD3YGL223C(-)--
    PMT2YAL023C(-)-
    ARV1YLR242C(-)(-)
    MNN5YJL186W(-)(-)
    FYV10YIL097W(-)(-)
    KRE33YNL132W(-)(-)
    OSH1YAR044W(-)(--)
    SBE22YHR103W(-)(--)
    FYV6YNL133C(--)(--)
β-1,6-Glucan elevated and β-1,3-glucan reduced
    MNN10YDR245W+--
    MNN9YPL050C+-
    YUR1YJL139C(+)(-)
    SHE4YOR035C(+)(--)
β-1,6-Glucan reduced and β-1,3-glucan elevated
    BIG1YHR101C---(+)
    KRE1YNL322C--(++)
    KRE6YPR159W--(++)
    ROT2YBR229C(-)(++)
  • Increase (I): +++, I > 100%; ++, 65 < I < 100; +, 45 < I < 65; (+), 25 < I < 45; (++), I < 25%. Decrease (D): ---, 85 < D < 100; --, 65 < D < 85; -, 45 < D < 65; (-), 25 < D < 45; (--), D < 25%.