TABLE 3

Genes whose deletion causes hypersensitivity to the K1 killer toxin (haploid or homozygous diploid death zone >115% of the wild type)

K1 killer toxin death zone (%)Calcofluor
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Gene nameORFDescription of gene productHaploidHeterozygousHomozygousHygromycin BSDS
1. Kinases, phosphatases, signal transduction (8 genes)
HOG1YLR113Wser/thr protein kinase of MAPK family196wt160wtwtS
PBS2YJL128CTyrosine protein kinase of the MAP kinase kinase family193wt179
SPS1YDR523Cser/thr protein kinase152wtNA
STE11YLR362Wser/thr protein kinase of the MEKK family139wtNAwtwtS
SSK1YLR006CTwo-component signal transducer135wt111RwtS
SSK2YNR031CMAP kinase kinase kinase of the HOG pathway130wt114
TPD3YAL016Wser/thr protein phosphatase 2 A, regulatory chain A122wtNA
DIG1YPL049CDownregulator of invasive growth and mating113wt118Rwtwt
2. Transcription (7 genes)
MED2YDL005CTranscriptional regulation mediator166wtNA
GAL11YOL051WRNA Pol II holoenzyme (SRB) subcomplex subunit158wt162SSS
SRB5YGR104CRNA Pol II holoenzyme (SRB) subcomplex subunit131wt124SSS
SRB2YHR041CRNA Pol II holoenzyme (SRB) subcomplex subunit124wt128
ITC1YGL133WSubunit of Isw2 chromatin remodeling complex121wt125
UME6YDR207CNegative transcriptional regulator118wt139SwtS
SW16YLR182WTranscription factor118wt119SSS
3. RNA processing (6 genes)
PRP18YGR006WU5 snRNA-associated protein148wt103wtwtwt
CBC2YPL178WSmall subunit of the nuclear cap-binding protein complex CBC139wt119Rwtwt
CDC40YDR364CRequired for mRNA splicing137wtNASSS
NSR1YGR159CNuclear localization sequence binding protein122wt116Swtwt
BRR1YPR057WInvolved in snRNP biogenesis120wt116wtwtwt
STO1YMR125WLarge subunit of the nuclear cap-binding protein complex CBC119wt124
4. Ribosomal and translation initiation proteins (18 + 2 genes)
RPS16BYDL083CRibosomal protein S16.e157wt116
RPS19BYNL302C40S small subunit ribosomal protein S19.e149wtNA
RPS24BYIL069C40S small subunit ribosomal protein S24.e149wt144
RPS24AYER074W40S small subunit ribosomal protein S24.e129wt142wtwtwt
RPS10AYOR293WRibosomal protein S10.e128112118Rwtwt
ASC1YMR116C40S small subunit ribosomal protein127wt130
RPS17AYML024WRibosomal protein S17.e.A127wt128wtwtwt
RPS11AYDR025WRibosomal protein S11.e126wt139
RPS23BYPR132W40S small subunit ribosomal protein S23.e126wt123
RPL13BYMR142C60S large subunit ribosomal protein124wt130
RPS16AYMR143WRibosomal protein S16.e121wt135wtwtwt
RPS0BYLR048W40S ribosomal protein p40 homolog B120wt125
RPS30BYOR182CSimilarity to human ubiquitin-like protein/ribosomal protein S30120wt120
RPL2BYIL018W60S large subunit ribosomal protein L8.e118111136
RPS4BYHR203CRibosomal protein S4.e.c8117wt114
RPS30AYLR287C-A40S small subunit ribosomal protein116wt114
RPL14AYKL006WRibosomal protein115wt145wtwtwt
RPS6BYBR181CRibosomal protein S6.e114115126
TIF3YPR163CTranslation initiation factor eIF4B154wt139wtwtS
YIF2YAL035WGeneral translation factor eIF2 homolog122wtNA
5. Protein modification or N-glycosylation (5 genes)
MNN9aYPL050CRequired for complex N-glycosylation152wt146SSS
ANP1aYEL036CRequired for protein glycosylation in the Golgi135wt129SSS
MAP1aYLR244CMethionine aminopeptidase, isoform 1134wt130SSS
MNN10aYDR245WSubunit of α-1,6-mannosyl transferase complex133wt136SSS
LAS21aYJL062WRequired for side-chain addition to GPI114wt119
6. Cellular polarity (5 genes)
BUD30aYDL151CK1 toxin hypersensitivity phenotype134wt107
BUD22YMR014WProtein with possible role in bud site polarity128wt144SSwt
BUD25YER014C-AProtein involved in bipolar budding123wt115SSS
BEM1YBR200WBud emergence mediator121wt114SSS
BUD27aYFL023WK1 toxin hypersensitivity phenotype115wt157
7. New FYV genes (8 genes)
FYV1aYDR024WK1 toxin hypersensitivity phenotype141wt138
FYV4YHR059WK1 toxin hypersensitivity phenotype127wt116
FYV5aYCL058CK1 toxin hypersensitivity phenotype125wt119
FYV6aYNL133CK1 toxin hypersensitivity phenotype123wt112
FYV7aYLR068WK1 toxin hypersensitivity phenotype120wt116wtwtwt
FYV8YGR196CK1 toxin hypersensitivity phenotype; similarity to Arp1p119wt121SSS
FYV10aYIL097WK1 toxin hypersensitivity phenotype116wt115wtSS
FYV12aYOR183WK1 toxin hypersensitivity phenotype114wt123wtSwt
8. Ungrouped or poorly characterized genes (12 genes)
CSF1aYLR087CRequired for normal growth rate and resistance to NaCl and H2O2140wt125SSS
VID21aYDR359CMutant impaired in fructose-1,6-bisphosphatase degradation139wt136SSS
ARV1aYLR242CLipid and sterol metabolism137wt123SSS
SEC66aYBR171WER protein-translocation complex subunit133wt144
PFD1YJL179WPrefoldin subunit 1133wt117SSS
CTF4YPR135WDNA-directed polymerase α-binding protein127wt112wtwtwt
SYG1YIL047CMember of the major facilitator superfamily126wt145
IWR1YDL115CHypothetical protein126wt110
YDJ1YNL064CMitochondrial and ER import protein121wt130
APL4YPR029CGamma-adaptin of clathrin-associated AP-1 complex116wt117wtSwt
HMO1YDR174WNonhistone protein115wt109
ADK1YDR226WAdenylate kinase, cytosolic112wt127SSS
  • Mutants with a wild-type phenotype in haploid or homozygous diploid background and those with a resistant phenotype in these backgrounds are not listed. NA, not available.

  • a Mutants with a β-glucan phenotype (see Table 5)