TABLE 2

Genes whose deletion causes resistance to the K1 killer toxin (haploid or homozygous diploid death zone <75% of the wild type)

K1 killer toxin death zone (%)Calcofluor
white
Gene nameORFDescription of gene productHaploidHeterozygousHomozygousHygromycin BSDS
1. Kinases, phosphatases, signal transduction (16 genes)
PHO80YOL001WCyclin that interacts with Pho85p protein kinase488563SSwt
PHO85YPL031CCyclin-dependent protein kinase56wt37RSS
PTP3YER075CProtein tyrosine phosphatase65wt63Rwtwt
CKA2YOR061WCasein kinase II α′ chain65wt63Rwtwt
SAC7aYDR389WGTPase-activating protein for Rho1p67wt85Swtwt
ELM1YKL048Cser/thr-specific kinase69wt58SwtS
PTC1YDL006WProtein serine/threonine phosphatase 2c71wt64
KIN3YAR018Cser/thr protein kinase72wt70
SAP155YFR040WSit4p-associated protein73wt62
SAP190YKR028WSit4p-associated protein74wt75
GPA2aYER020WGuanine nucleotide-binding regulatory protein74wt83wtwtwt
RRD1YIL153WPhosphotyrosyl phosphatase activator75wt69
MKS1YNL076WNegative regulator of Ras-cAMP pathway82wt83
GLC8YMR311CRegulatory subunit for ser/thr phosphatase Glc7p83wt69
PHO86YJL117WInorganic phosphate transporter83wt70
SAL6YPL179WPhosphoprotein phosphatase85wt67
2. Transcription (21 genes)
SSN6YBR112CGeneral repressor of transcription0810
TUP1YCR084CGeneral transcription repressor0830
CTK3YML112WCTD kinase, gamma subunit; RNA Pol II regulation0wt0
SNF12YNR023WComponent of SWI/SNF global transcription activator complex0wtNA
CCR4YAL021CTranscriptional regulator42wt57Rwtwt
POP2YNR052CRequired for glucose derepression60wt70
ELP4YPL101WSubunit of RNA Pol II elongator complex68wt76Swtwt
ADA2YDR448WGeneral transcriptional adaptor or coactivator69wt63
CTK1YKL139WCTD kinase, alpha subunit; RNA Pol II regulation70wt58
ACE2YLR131CMetallothionein expression activator71wt75RRwt
SIR4YDR227WSilencing regulatory and DNA-repair protein72wtNAwtwtwt
IK13YLR384CElongator complex; RNA Pol II-associated protein73wt75
SSN3YPL042CCyclin-dependent CTD kinase73wt78
SIR1YKR101WSilencing regulatory protein73wt80
FUN30YAL019WMember of the Snf2p family of ATP-dependent DNA helicases76wt72
EPL6YMR312WSubunit of RNA Pol II elongator complex79wt58
ELP3YPL086CHistone and other protein acetyltransferase80wt65
SPT10YJL127CTranscription regulatory protein80wt70
SAP30YMR263WComponent of the Rpd3p-Sin3p histone deacetylase complex82wt65
SIG1YER068WTranscriptional repressor83wt32SSS
TAF14YPL129WTFIIF subunit (transcription initiation factor), 30 kD85wt56SSS
3. Actin organization (7 genes)
ARC18YLR370CSubunit of the Arp2/3 complex0wt0
VRP1aYLR337CVerprolin, involved in cytoskeletal organization0wt0RSS
LAS17aYOR181WComponent of actin cortical patches0wtNASSS
SAC6YDR129CActin filament bundling protein, fimbrin49wt57SSwt
VIP1YLR410WInvolved in cortical actin function62wt72
NBP2YDR162CNap1p-binding protein708573
SLA2YNL243WCytoskeleton assembly control protein71wtNASSS
4. Lipid/sterol synthesis (9 genes)
PLC1YPL268WLipid, fatty-acid, and sterol metabolism and signal transduction0wt0
ERG2YMR202WC-8 sterol isomerase55wt51SSS
ERG28YER044CProtein involved in synthesis of ergosterol57wt52SSS
IPK1aYDR315CIP5 kinase66wt79
ERG4aYGL012WSterol C-24 reductase73wt70SSS
DEP1YAL013WRegulator of phospholipid metabolism73wt72
OSH1aYAR044WSimilarity to human oxysterol binding protein (OSBP)77wt72
SUR4aYLR372WSterol isomerase, fatty acid elongase83wt74
LCB83YJL134WSphingoid base-phosphate phosphatase, putative regulator84wt84
5. Secretion/endocytosis (23 genes)
ERV41aYML067CCOPII ER-Golgi vesicle protein0wt0
CLC1YGR167WClathrin light chain0wt0SSS
KRE11aYGR166WTRAPPII Golgi vesicular transport protein0wt0SwtS
LUV1YDR027CInvolved in protein sorting in the late Golgi0wt0
SAC1aYKL212WRole in Golgi function and actin cytoskeleton organization0wt0SSS
SHE4aYOR035CRequired for mother-cell-specific gene expression and for endocytosis0wt0SSwt
SWA2YDR320CClathrin-binding protein required for normal clathrin function0wt0SSS
RCY1YJL204CF-box protein involved in endocytic membrane traffic0wt0SSS
SAC2YDR484WSuppressor of actin mutation, involved in vesicular transport0wtNA
ERV46YAL042WVesicular transport between Golgi and ER38wt42wtwtwt
VPS74YDR372CVacuolar protein sorting53wt70SSS
VAC8aYEL013WRequired for vacuole inheritance and vacuole targeting557965wtwtwt
CHC1YGL206CClathrin heavy chain59wt0
COD3aYGL223CComplex with Sec34p-Sec35p involved in vesicle transport to the Golgi59wt69SSS
YPT6YLR262CGTP-binding protein of the rab family69wt73
ERV14aYGL054CStrong similarity to D. melanogaster cni protein71wt76
COD2YNL041CComplex with Sec34p-Sec35p involved in vesicle transport to the Golgi73wt73
ARL3YPL051WRequired for transport from ER to Golgi and Golgi to vacuoles73wt80
VPS61aYDR136CVacuolar protein sorting78wt67
VPS67aYKR020WVacuolar protein sorting78wt68
VPS1YKR001CMember of the dynamin family of GTPases; vacuolar sorting protein79wt68
PEP3aYLR148WVacuolar protein sorting84wt57SSS
END3aYNL084CRequired for endocytosis and cytoskeletal organization85wt70
6. Protein glycosylation (13 genes)
CWH41YGL027CER glucosidase I0wt0SSwt
GDA1YEL042WGuanosine diphosphatase0wt0SSS
ROT2aYBR229CER glucosidase II, catalytic subunit0wt0SSS
YUR1aYJL139CMannosyltransferase0wt0
OST3YOR085WOligosaccharyltransferase gamma subunit09233SSS
PMT1YDL095WMannosyltransferase0wt68
KRE2YDR483Wα-1,2-Mannosyltransferase0wtNA
CWH8YGR036CDolichol-P-phosphatase0wt75SSS
ALG6YOR002WGlucosyltransferase53wt72SwtS
ALG8YOR067CGlucosyltransferase61wt79SwtS
MNN5aYJL186WPutative mannosyltransferase64wt81
ALG5YPL227CDolichol-P-glucose synthetase70wt79SwtS
PMT2aYAL023CMannosyltransferase77wt56SwtS
7. Protein modification or degradation (6 genes)
MAK10YEL053CSubunit of Mak3p-10p-31p N-terminal acetyltransferase56wt73RwtS
KEX1aYGL203CCarboxypeptidase (YSC-α)57wt70SRS
DOC1YGL240WComponent of the anaphase-promoting complex67wt80
BTS1aYPL069CGeranylgeranyl diphosphate synthase72wt65
VPS27aYNR006WVacuolar protein sorting-associated protein80wt67
YPS7YDR349CGPI-anchored aspartic protease83wt58SSS
8. Cell wall organization (8 genes)
KRE1aYNL322CGPI-anchored plasma membrane protein0620SSS
KRE6aYPR159Wcis-Golgi glucanase-like protein0720SSS
FKS1aYLR342W1,3-β-d-Glucan synthase-associated protein0850SSwt
CNE1aYAL058WCalnexin, regulation of secretion and cell wall organization0wt0SSS
SMI1aYGR229Cβ-1,3-Glucan synthesis protein0wt0SSS
SBE22aYHR103WSimilarity to budding protein Sbe2p779180
SCW4YGR279CGlucanase gene family member84wt72
ECM30YLR436CInvolved in cell wall biogenesis and architecture84wt73
9. Mitochondrial, respiratory, and ATP metabolism (17 genes)
ILM1YJR118CPossibly involved in mitochondrial DNA maintenance0wt0
ATP2YJR121WF1F0-ATPase complex, F1 β-subunit0wt65
IMG2YCR071CRequired for integrity of mitochodrial genome0wt75
NAM2YLR382CLeucine-tRNA ligase precursor, mitochondrial547879
OCT1YKL134CMitochondrial intermediate peptidase60wt79
OXA1YER154WCytochrome oxidase biogenesis protein65wt83
DIA4YHR011WMay be involved in mitochondrial function70wt60
MRPL8YJL063CRibosomal protein L17, mitochondrial70wt73
MGM101YJR144WMitochondrial genome maintenance protein70wt76
MRPL38YKL170WRibosomal protein of the large subunit, mitochondrial71wt81
MRPL33YMR286WRibosomal protein of the large subunit, mitochondrial72wt77
MMM1YLL006WRequired for mitochondrial shape and structure73wt66
ISA2YPR067WMitochondrial protein required for iron metabolism73wt72
MRPL27YBR282WRibosomal protein YmL27 precursor, mitochondrial74wt74
IMG1YCR046CRibosomal protein, mitochondrial81wt73
ATP15YPL271WF1F0-ATPase complex, F1 epsilon subunit84wt55wtSS
BCS1YDR375CMitochondrial protein, involved in the assembly of cytb cl complex85wt81
10. Ungrouped genes (16 genes)
SOD1YJR104CCopper-zinc superoxide dismutase0wt0
UTH1aYKR042WInvolved in the aging process0wt0wtwtwt
DRS2YAL026CP-type calcium-ATPase0wt56wtSS
NPL3YDR432WNucleolar protein31wt0SSS
LEM3YNL323WProtein with similarity to Ycx1p, mutant is sensitive to brefeldin A44wt45SwtS
GLY1aYEL046Cl-threonine aldolase, low specificity45wt55SSS
ATS1YAL020Cα-Tubulin suppressor71wt78
YTA7YGR270W26S proteasome subunit74wt80
DFG5YMR238WRequired for filamentous growth, cell polarity, and cellular elongation74wt80
THP1aYOL072WHypothetical protein76700SSS
CDC50YCR094WCell division cycle mutant77wt62
NEW1YPL226WMember of the nontransporter group of the ABC superfamily77wt66
LYS7YMR038CCopper chaperone for superoxide dismutase Sod1p78wt62
FPS1aYLL043WGlycerol channel protein79wt65SSS
UTR1YJR049CAssociated with ferric reductase activity84wt70
PEX12YMR026CRequired for biogenesis of peroxisomes—peroxin84wt72
11. Genes of currently unknown function or poorly characterized (19 genes)
BUD14YAR014CProtein of unknown function54wt47SSS
YFR043CHypothetical protein55wt70wtwtwt
aYKL037WWeak similarity to Caenorhabditis elegans ubc-2 protein59wt67SSS
aYGL007WQuestionable ORF63wt70
aYNL213CHypothetical protein67wtNARSwt
PIN4aYBL051CSimilarity to Schizosaccharomyces pombe Z66568_C protein70wt70
YOR154WSimilarity to hypothetical Arabidopsis thaliana proteins71wt84
YLR270WStrong similarity to YOR173w71wtNARwtwt
YNL063WSimilarity to S-adenosyl methionine-dependent methyl-transferase73wt76
MON2YNL297CUnknown function, sensitive to monensin and brefeldin A80wt70
TOS1YBR162CProtein with similarity to Aga1p81wt66
YER140WHypothetical protein82wt66
SYS1aYJL004CMulticopy suppressor of ypt682wt74
YLL007CHypothetical protein83wt67
EAF6aYJR082CHypothetical protein83wt76
YDR126WSimilarity to hypothetical protein YLR246w and YOL003c84wt66
YGR263CWeak similarity to Escherichia coli lipase like enzyme84wt68
KRE27YIL027CK1 toxin resistance phenotype; has a hydrophobic domain858054
YBR284WSimilarity to AMP deaminase85wt70
  • Mutants not listed are those with a wild-type phenotype in haploid or homozygous diploid background and those that are hypersensitive (Table 3) or haploinsufficient (Table 4). R, resistant; S, hypersensitive; wt, wild type to Calcofluor white, Hygromycin B, and SDS. NA, not available.

  • a Indicates mutants with a β-glucan phenotype (see Table 5).