TABLE 1

Repair and rearrangements after a DSB at the URA3::actin intron::HO cut site locus

Chromosomal rearrangements resulting in 5-FOA resistance
Genetic
background
Frequency of
survival per
plated cell
Frequency of
5-FOA resistance
per plated cell
Insertions
(%)
Gene
conversions
(%)
Small
deletionsa
(%)
Large
deletionsb
(%)
Translocations
and inversions
(%)
Unknownc
(%)
Nd
WT1.90 × 10-27.51 × 10-440472173046
rad524.51 × 10-35.83 × 10-552195121242
yku808.39 × 10-33.61 × 10-578121150
rad52 yku801.49 × 10-41.05 × 10-5587879
  • a Small deletions are between 117 and 1000 bp.

  • b Large deletions are >1 kb.

  • c The unknown events are the 5-FOA-resistant clones for which no PCR product was obtained using all means described (WT, rad52, and rad52 yku80).

  • d N refers to the nos. of independent 5-FOA-resistant colonies from which genomic DNA was obtained and analyzed.