TABLE 3

Correlation between male fertility of rf2a-m9390/rf2a-R213 plants and the methylation status of Mu1 transposons within their genomes and within rf2a-m9390

No. of plants
All Mu1Mu1 from rf2a-m9390a
FamiliesPhenotypesbMethylatedcHypomethylateddMethylatedeHypomethylatedf
96 2209S0403
96 2214“S”0202
96 2218“F”0000
F11252
92 2148S1111
“S”0202
“F”2020
F2020
  • a Not all plants were subjected to this analysis.

  • b Plant phenotypes: F, fully male fertile; “F,” more than one-half of the anthers exerted; “S,” some anthers exerted; S, completely male sterile with no anthers exerted.

  • c Plants were scored as “methylated” if they contained Mu1-hybridizing fragments >1.34 kb and had no or few 1.34-kb Mu1-hybridizing DNA fragments (e.g., see F in Figure 2A).

  • d Plants were scored as “hypomethylated” if prominent 1.34-kb Mu1-hybridizing DNA fragments were identified (e.g., see S in Figure 2A).

  • e Plants were scored as “methylated” if no or few rf2a-hybridizing 1.1-kb DNA fragments and strong rf2a-hybridizing 2.4-kb and/or 2.8-kb DNA fragments were observed (e.g., see F in Figure 2B).

  • f Plants were scored as “hypomethylated” if a strong rf2a-hybridizing 1.1-kb DNA fragment and weak 2.4-kb and/or 2.8-kb fragments were observed (e.g., see S in Figure 2B).