Reactivation of suppressed rf2a-m alleles

FamiliesAllelesCrossesNo. of fertile progenyNo. of sterile progenyReactivation ratioa (%)
97 5432 rf2a-m9390 (T) rf2a-m/rf2a-m (F)b × rf2a-R213/Rf2a (Mu on)362071
97 5433-34 rf2a-m9390 Same as above1142130
96g 6104-05 rf2a-m9390 (T) rf2a-m/rf2a-m (F) self2000
97 5435-36 rf2a-m8110 (T) rf2a-R213/rf2a-R213 (Mu on) × rf2a-m/rf2a-R213 (F)189568
97 5437 rf2a-m8110 Same as above1980
97 5446 rf2a-m8110 (T) rf2a-m/rf2a-R213 (Mu on) × rf2a-m/rf2a-m (F)555048
98 6336 rf2a-m8110 (T) rf2a-m/rf2a-m (F) self1500

Mu-active lines (indicated as “Mu on”) were crossed to plants carrying suppressed rf2a-m alleles. The resulting progenies were scored for male-fertile plants. Each row in the table is from a single reactivation cross. Mu-active lines are progenies of Mu-active parents confirmed by seedling tests.

  • a The reactivation ratio was calculated by dividing the number of male-sterile plants by the number of male-sterile plants expected if the rf2a-m alleles displayed 100% penetrance. This formula was adjusted to account for the segregation of homozygous rf2a-R213 plants in families 97 5435–36 and 97 5437.

  • b (F) plants are homozygous for rf2a mutants but male fertile.