TABLE 1

Comparison of the penetrance of suppressible and nonsuppressible rf2a-m alleles

No. of plants
F + “F”S + “S”Penetrancea (%)
FamiliesAllelesRf2a/rf2arf2a-m/rf2aRf2a/rf2arf2a-m/rf2aTotal no.
92 2123 rf2a-m8110 16 3 072670
92 2148 rf2a-m9390 4 6 041440
92 2126 rf2a-m8122 4 0 01418100
92 2153 rf2a-m9437 13 0 01225100

Random plants from the cross (T) rf2a-m/Rf2a-Ky21 × rf2a-R213/rf2a-R213 were genotyped via PCR using rf2a-specific and Mu-specific primers (materials and methods). The number of suppressed mutant plants in each family is underlined. Plant phenotypes: F, fully male fertile; “F,” more than one-half of the anthers exerted; “S,” some anthers exerted; S, completely male sterile with no anthers exerted.

  • a Penetrance was calculated as the ratio of sterile plants (S + “S”) with the genotype rf2a-m/rf2a-R213 to the total number of plants with the genotype rf2a-m/rf2a-R213. On the basis of a chi-square homogeneity test the ratios of fertile rf2a-m/rf2a plants in families 92 2123 and 92 2148 are significantly different at 95% confidence level from those in families 92 2126 and 92 2153.